Alternative pathways for angiotensin II production as an important determinant of kidney damage in endotoxemia

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dc.contributor.authorRosa, Rodolfo Mattar [UNIFESP]
dc.contributor.authorColucci, Juliana Almada [UNIFESP]
dc.contributor.authorYokota, Rodrigo [UNIFESP]
dc.contributor.authorMoreira, Roseli Peres [UNIFESP]
dc.contributor.authorAragao, Danielle Sanches [UNIFESP]
dc.contributor.authorRibeiro, Amanda Aparecida [UNIFESP]
dc.contributor.authorArita, Danielle Yuri [UNIFESP]
dc.contributor.authorMizuno Watanabe, Ingrid Kazue [UNIFESP]
dc.contributor.authorPalomino, Zaira [UNIFESP]
dc.contributor.authorCunha, Tatiana Sousa [UNIFESP]
dc.contributor.authorCasarini, Dulce Elena [UNIFESP]
dc.date.accessioned2019-07-22T15:46:45Z
dc.date.available2019-07-22T15:46:45Z
dc.date.issued2016
dc.description.abstractSepsis is an uncontrolled systemic inflammatory response against an infection and a major public health issue worldwide. This condition affects several organs, and, when caused by Gram-negative bacteria, kidneys are particularly damaged. Due to the importance of renin-angiotensin system (RAS) in regulating renal function, in the present study, we aimed to investigate the effects of endotoxemia over the renal RAS. Wistar rats were injected with Escherichia coli lipopolysaccharide (LPS) (4 mg/kg), mimicking the endotoxemia induced by Gram-negative bacteria. Three days after treatment, body mass, blood pressure, and plasma nitric oxide (NO) were reduced, indicating that endotoxemia triggered cardiovascular and metabolic consequences and that hypotension was maintained by NO-independent mechanisms. Regarding the effects in renal tissue, inducible NO synthase (iNOS) was diminished, but no changes in the renal level of NO were detected. RAS was also highly affected by endotoxemia, since renin, angiotensin-converting enzyme (ACE), and ACE2 activities were altered in renal tissue. Although these enzymes were modulated, only angiotensin (ANG) II was augmented in kidneysen
dc.description.abstractANG I and ANG 1-7 levels were not influenced by LPS. Cathepsin G and chymase activities were increased in the endotoxemia group, suggesting alternative pathways for ANG II formation. Taken together, our data suggest the activation of noncanonical pathways for ANG II production and the presence of renal vasoconstriction and tissue damage in our animal model. In summary, the systemic administration of LPS affects renal RAS, what may contribute for several deleterious effects of endotoxemia over kidneys.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Div Nephrol, Dept Med, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Ciencia & Tecnol, Sci & Technol Dept, Sao Jose Dos Campos, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Div Nephrol, Dept Med, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Ciencia & Tecnol, Sci & Technol Dept, Sao Jose Dos Campos, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/04940-2, 2010/51904-9]
dc.description.sponsorshipIDFAPESP:2011/04940-2
dc.description.sponsorshipID2010/51904-9
dc.format.extentF496-F504
dc.identifierhttp://dx.doi.org/10.1152/ajprenal.00121.2014
dc.identifier.citationAmerican Journal Of Physiology-Renal Physiology. Bethesda, v. 311, n. 3, p. F496-F504, 2016.
dc.identifier.doi10.1152/ajprenal.00121.2014
dc.identifier.issn1931-857X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51070
dc.identifier.wosWOS:000384977500002
dc.language.isoeng
dc.publisherAmer Physiological Soc
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectrenin-angiotensin systemen
dc.subjectendotoxemiaen
dc.subjectacute kidney injuryen
dc.titleAlternative pathways for angiotensin II production as an important determinant of kidney damage in endotoxemiaen
dc.typeinfo:eu-repo/semantics/article
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