Time-dependent up-regulation of Ca(2+) channels in vas deferens of newborn rats fed with breast milk of mothers under treatment with nifedipine

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dc.contributor.authorValera, Adriana Gonzalez [UNIFESP]
dc.contributor.authorVerde, Luciana Ferreira [UNIFESP]
dc.contributor.authorLafayette, Simone Sette Lopes [UNIFESP]
dc.contributor.authorVerreschi, Ieda Therezinha do Nascimento [UNIFESP]
dc.contributor.authorCaricati-Neto, Afonso [UNIFESP]
dc.contributor.authorJurkiewicz, Aron [UNIFESP]
dc.contributor.authorJurkiewicz, Neide Hyppolito [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.date.accessioned2016-01-24T13:51:58Z
dc.date.available2016-01-24T13:51:58Z
dc.date.issued2008-12-03
dc.description.abstractOur aim was to check for calcium channel maturation and regulation on newborn rats during breastfeeding by mothers treated with the L-type calcium channel blocker nifedipine. Contractions by KCl and radioligand binding techniques were used to verify if Call Channels are modified in rat vas deferens of 40-day old litters that were breastfed by mothers injected daily with nifedipine during nursery. Injections were applied in the beginning (1st until 8th day), middle (9th until 16th day), or end (17th until 24th day) of nursery, to verify the period of highest susceptibility of newborn to nifedipine receptor regulation. Contractile responses revealed that only after the middle period of treatment of mothers the maximal effects (E(max)) induced in pups by KCl were increased by about 35%, without changes of apparent affinity (pD(2)). Additionally, binding studies with [(3)H] Isradipine in cell membrane preparations showed a greater density (B(max)) of Ca(2+) channels by about 55%, without changes of affinity (K(d)). Changes were not detected after treatment of mothers in the beginning or end of breastfeeding. in addition, in vas deferens of 60-day old litters, the E(max) returned to control values, showing that changes were not persistent. Moreover, body and vas deferens weights and blood testosterone of newborn were never changed. the histology of mammary gland was similar for treated and control mothers, suggesting a stable milk production. It is concluded that nifedipine treatment of mothers, if made during the 9th to 16th day of lactation, produced a short lasting reversible up-regulation of L-type Ca(2+) channels. (C) 2008 Published by Elsevier B.V.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pharmacol, BR-04034970 São Paulo, SP, Brazil
dc.description.affiliationUniv Fed Pernambuco, Dept Pharmacol, Recife, PE, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, BR-04034970 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pharmacol, BR-04034970 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, BR-04034970 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent131-136
dc.identifierhttps://dx.doi.org/10.1016/j.ejphar.2008.09.022
dc.identifier.citationEuropean Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 599, n. 1-3, p. 131-136, 2008.
dc.identifier.doi10.1016/j.ejphar.2008.09.022
dc.identifier.issn0014-2999
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/31096
dc.identifier.wosWOS:000261157500021
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofEuropean Journal of Pharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBreastfeedingen
dc.subjectNifedipineen
dc.subjectCalcium channelen
dc.subjectSmooth muscleen
dc.subjectVas deferensen
dc.subjectNewborn raten
dc.titleTime-dependent up-regulation of Ca(2+) channels in vas deferens of newborn rats fed with breast milk of mothers under treatment with nifedipineen
dc.typeinfo:eu-repo/semantics/article
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