Intrauterine food restriction alters the expression of uncoupling proteins in brown adipose tissue of rat newborns

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dc.contributor.authorSouza, Thais L. V. [UNIFESP]
dc.contributor.authorCoelho, Carolina T. [UNIFESP]
dc.contributor.authorGuimarães, Paola Bianchi [UNIFESP]
dc.contributor.authorGoto, Eduardo M. [UNIFESP]
dc.contributor.authorSilva, Sylvia Maria A. [UNIFESP]
dc.contributor.authorSilva, Jose Antonio
dc.contributor.authorNunes, Maria Tereza
dc.contributor.authorIhara, Silvia S. M. [UNIFESP]
dc.contributor.authorLuz, Jacqueline [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Nove Julho
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:17:52Z
dc.date.available2016-01-24T14:17:52Z
dc.date.issued2012-02-01
dc.description.abstractA previous study from our laboratory showed that maternal food restriction (MFR) delays thermoregulation in newborn rats. in neonates brown adipose tissue (BAT) is essential for thermogenesis due to the presence of uncoupling proteins (UCPs). the aim of this study was to evaluate the influence of MFR on the UCPs mRNA and protein expression in BAT and skeletal muscle (SM) of the newborn rat. Female Wistar EPM-1 control rats (CON) received chow ad libitum during pregnancy, whereas food-restricted dams (RES) received 50% of the amount ingested by CON. Fifteen hours after birth, the litters were weighed and sacrificed. Blood was collected for hormonal analysis. BAT and SM were used for determination of UCPs mRNA and protein expression, and Ca2+-ATPase sarcoplasmic reticulum (SERCA1). RES pups showed a significant reduction in body weight and fat content at birth. MFR caused a significant increase in the expression of UCP1 and UCP2 in BAT, without changes in UCP3 and SERCA1 expression in BAT and SM. No differences between groups were found for leptin, T4 and glucose levels. RES pups showed increased insulin and decreased T3 levels. the delay in development of thermoregulation previously described in RES animals appears not to result from impairment in thermogenesis, but from an increase in heat loss, since MFR caused low birth weight in pups, leading to greater surface/volume ratio. the higher expression of UCP1 and UCP2 in BAT suggests a compensatory mechanism to increased thermogenesis. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Physiol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pathol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv Nove Julho, Dept Rehabil Sci, UNINOVE, BR-05001100 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Physiol & Biophys, BR-05508000 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Physiol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pathol, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 04/14749-4
dc.format.extent138-143
dc.identifierhttp://dx.doi.org/10.1016/j.jtherbio.2011.12.002
dc.identifier.citationJournal of Thermal Biology. Oxford: Pergamon-Elsevier B.V., v. 37, n. 2, p. 138-143, 2012.
dc.identifier.doi10.1016/j.jtherbio.2011.12.002
dc.identifier.issn0306-4565
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34589
dc.identifier.wosWOS:000300918000006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Thermal Biology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBody temperatureen
dc.subjectMaternal food restrictionen
dc.subjectUndernutritionen
dc.subjectThermoregulationen
dc.subjectUncoupling proteinsen
dc.titleIntrauterine food restriction alters the expression of uncoupling proteins in brown adipose tissue of rat newbornsen
dc.typeinfo:eu-repo/semantics/article
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