Avaliação da atividade fagocítica e microbicida de macrófagos alveolares de ratos de baixo peso ao nascer
Data
2023-01-12
Autores
Negreiros, Náthani Gabrielly Silva 
Orientadores
Landgraf, Richardt Gama
Tipo
Trabalho de conclusão de curso
Título da Revista
ISSN da Revista
Título de Volume
Resumo
A restrição alimentar materna durante a gravidez induz adaptações fetais morfológicas
e metabólicas e predispõe a doenças metabólicas, incluindo diabetes e hipertensão,
na prole. Distúrbios metabólicos também podem ser induzidos pelo desequilíbrio de
fatores pró e anti-inflamatórios, como observado na sepse. Em estudos anteriores,
nosso grupo demonstrou que a restrição alimentar durante a gestação resultou em
baixo peso ao nascer, hipocelularidade na medula óssea e no sangue periférico,
redução na migração de leucócitos e baixa resposta inflamatória em ratas Wistar com
12 semanas. O objetivo deste estudo foi avaliar a capacidade fagocítica e microbicida
de macrófagos alveolares, de animais que sofreram restrição alimentar intrauterina.
Para isso, os animais foram acasalados, ratos Wistar fêmeas e machos da mesma
idade (12 a 16 semanas de idade). O dia 1 da gestação foi definido como o dia em
que os espermatozoides foram detectados no esfregaço vaginal. Após a confirmação
da ocorrência do acasalamento, as ratas foram divididas aleatoriamente em 2 grupos:
Controles - dieta e água ad libitum; Restritos - 50% de restrição alimentar e água ad
libitum. Com 12 semanas de idade, ratos machos descendentes de ambos os grupos
foram eutanasiados e os macrófagos alveolares incubados com Saccharomyces
cerevisiae, na proporção de 4:1, por 4 horas. Para avaliar a atividade microbicida foi
feita a contagem das unidades formadoras de colônia (UFC) dessas células com
Saccharomyces cerevisiae. Observamos que as mães que sofreram restrição
alimentar não apresentaram ganho de peso durante o período gestacional e os filhotes
nasceram com baixo peso e tamanho reduzido. Após o 20º dia de vida, os filhotes de
baixo peso (restrito) igualaram-se ao peso dos filhotes de peso normal (controle),
superando a diferença de massa corporal entre os grupos. O grupo restrito apresentou
menor atividade fagocítica e microbicida quando comparado ao grupo controle. Os
resultados obtidos até o momento indicam que a menor capacidade fagocitária e
microbicida dos macrófagos de ratos de baixo peso ao nascer pode estar contribuindo
para o desenvolvimento de doenças.
Maternal food restriction during pregnancy induces morphological and metabolic fetal adaptations and predisposes to metabolic diseases including diabetes and hypertension, in offspring. Metabolic disturbances can also be induced by the imbalance of pro and anti-inflammatory factors, as observed in sepsis. In previous studies, our group demonstrated that food restriction during pregnancy resulted in low birth weight, hypocellularity in bone marrow and peripheral blood, reduction in leukocyte migration, and a defective inflammatory response in Wistar rats at 12 weeks. The aim of this study was to evaluate the phagocytic and microbicidal capacity of macrophages of maternal malnutrition during the pregnancy, in adult offspring. The objective was to evaluate the phagocytic and microbicidal capacity of alveolar macrophages from animals that underwent intrauterine food restriction. Timed mating was carried out in age-matched (12- to 16-wk-old) female and male Wistar rats. Day 1 of the pregnancy was defined as the day on which spermatozoa were detected in the vaginal smear. After confirmation that mating had occurred, female rats were randomly divided into 2 groups: control - diet and water ad libitum; restricted - 50% food restriction and water ad libitum. At 12 weeks of age, male offspring of both groups were euthanized and the alveolar macrophages were incubated with Saccharomyces cerevisiae, in a 4:1 ratio, for 4 hours. To evaluated microbicidal activity was count of colony forming units (CFU) these cells with Saccharomyces cerevisiae. We observed that mothers who suffered dietary restriction did not present weight gain during the gestational period and the pups were born with low weight and reduced size. After the 20th day of life, the low birth weight (restricted) offspring equaled the weight of the normal birth weight (control) pups, overcoming the difference in body mass between the groups. The restricted group showed less phagocytic and microbicidal activity when compared to the control group. The results obtained so far indicate that the lower phagocytic and microbicidal capacity of macrophages from restricted group rats could compromises immune response and increases susceptibility to infections.
Maternal food restriction during pregnancy induces morphological and metabolic fetal adaptations and predisposes to metabolic diseases including diabetes and hypertension, in offspring. Metabolic disturbances can also be induced by the imbalance of pro and anti-inflammatory factors, as observed in sepsis. In previous studies, our group demonstrated that food restriction during pregnancy resulted in low birth weight, hypocellularity in bone marrow and peripheral blood, reduction in leukocyte migration, and a defective inflammatory response in Wistar rats at 12 weeks. The aim of this study was to evaluate the phagocytic and microbicidal capacity of macrophages of maternal malnutrition during the pregnancy, in adult offspring. The objective was to evaluate the phagocytic and microbicidal capacity of alveolar macrophages from animals that underwent intrauterine food restriction. Timed mating was carried out in age-matched (12- to 16-wk-old) female and male Wistar rats. Day 1 of the pregnancy was defined as the day on which spermatozoa were detected in the vaginal smear. After confirmation that mating had occurred, female rats were randomly divided into 2 groups: control - diet and water ad libitum; restricted - 50% food restriction and water ad libitum. At 12 weeks of age, male offspring of both groups were euthanized and the alveolar macrophages were incubated with Saccharomyces cerevisiae, in a 4:1 ratio, for 4 hours. To evaluated microbicidal activity was count of colony forming units (CFU) these cells with Saccharomyces cerevisiae. We observed that mothers who suffered dietary restriction did not present weight gain during the gestational period and the pups were born with low weight and reduced size. After the 20th day of life, the low birth weight (restricted) offspring equaled the weight of the normal birth weight (control) pups, overcoming the difference in body mass between the groups. The restricted group showed less phagocytic and microbicidal activity when compared to the control group. The results obtained so far indicate that the lower phagocytic and microbicidal capacity of macrophages from restricted group rats could compromises immune response and increases susceptibility to infections.
Descrição
Citação
NEGREIROS, Náthani Gabrielly Silva. Avaliação da atividade fagocítica e microbicida de macrófagos alveolares de ratos de baixo peso ao nascer. 2023. 30 f. Trabalho de conclusão de curso (Graduação em Ciências) - Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, 2023.