Surface chemistry and spectroscopy studies on 1,4-naphthoquinone in cell membrane models using Langmuir monolayers

Página do item simplificado
dc.contributor.authorHussein, Nadia [UNIFESP]
dc.contributor.authorLopes, Carla Cristina [UNIFESP]
dc.contributor.authorPernambuco Filho, Paulo Castanho de Almeida [UNIFESP]
dc.contributor.authorCarneiro, Bruna R. [UNIFESP]
dc.contributor.authorCaseli, Luciano [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:32:00Z
dc.date.available2016-01-24T14:32:00Z
dc.date.issued2013-07-15
dc.description.abstractInvestigating the role of drugs whose pharmaceutical activity is associated with cell membranes is fundamental to comprehending the biochemical processes that occur on membrane surfaces. in this work, we examined the action of 1,4-naphthoquinone in lipid Langmuir monolayers at the air-water interface, which served as a model for half of a membrane, and investigated the molecular interactions involved with tensiometry and vibrational spectroscopy. the surface pressure-area isotherms exhibited a noticeable shift to a lower area in relation to 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dihexadecanoyl-sn-glycero-3-phospho-L-serine (DPPS) lipid monolayers, which indicated a disruption of the monolayer structure and solubilisation of the lipids towards the aqueous subphase. To better correlate to the action of this drug in biological membrane events, cell cultures that represented tumorigenic and non-tumorigenic cells were spread onto the air-water interface, and 1,4-naphthoquinone was then incorporated. While only slight changes were observed in the non-tumorigenic cells upon drug incorporation, significant changes were observed in the tumorigenic cells, on which the organisation of the Langmuir monolayers was disrupted as evidenced by tensiometry and vibrational spectroscopy. This work then shows that this drug interacts preferentially for specific surfaces. in simplified models, it has a higher effect for the negative charged DPPS rather than the zwitterionic DPPC; and for complex cell cultures, 1,4-naphthoquinone presents a more significant effect for that representing tumorigenic cells. (c) 2013 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Diadema, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent300-306
dc.identifierhttp://dx.doi.org/10.1016/j.jcis.2013.04.005
dc.identifier.citationJournal of Colloid and Interface Science. San Diego: Academic Press Inc Elsevier Science, v. 402, p. 300-306, 2013.
dc.identifier.doi10.1016/j.jcis.2013.04.005
dc.identifier.fileWOS000319540400039.pdf
dc.identifier.issn0021-9797
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36540
dc.identifier.wosWOS:000319540400039
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Colloid and Interface Science
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBiomembranes modelsen
dc.subject1,4-Naphthoquinoneen
dc.subjectLangmuir monolayersen
dc.subjectPM-IRRASen
dc.titleSurface chemistry and spectroscopy studies on 1,4-naphthoquinone in cell membrane models using Langmuir monolayersen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000319540400039.pdf
Tamanho:
643.05 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
Em verificação - Geral