Reduced insulin secretion function is associated with pancreatic islet redistribution of cell adhesion molecules (CAMs) in diabetic mice after prolonged high-fat diet

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dc.citation.issue1
dc.citation.volume146
dc.contributor.authorFalcao, Viviane Tannuri F. L.
dc.contributor.authorMaschio, Daniela A.
dc.contributor.authorFontes, Camila Calvo de [UNIFESP]
dc.contributor.authorOliveira, Ricardo B.
dc.contributor.authorSantos-Silva, Junia C.
dc.contributor.authorSoares Almeida, Anna Carolina
dc.contributor.authorVanzela, Emerielle C.
dc.contributor.authorCartaxo, Maria Tereza
dc.contributor.authorCarvalho, Carolina Prado de França [UNIFESP]
dc.contributor.authorCollares-Buzato, Carla Beatriz
dc.coverageNew York
dc.date.accessioned2020-08-14T13:44:28Z
dc.date.available2020-08-14T13:44:28Z
dc.date.issued2016
dc.description.abstractIntercellular junctions play a role in regulating islet cytoarchitecture, insulin biosynthesis and secretion. In this study, we investigated the animal metabolic state as well as islet histology and cellular distribution/expression of CAMs and F-actin in the endocrine pancreas of C57BL/6/JUnib mice fed a high-fat diet (HFd) for a prolonged time period (8 months). Mice fed a HFd became obese and type 2 diabetic, displaying significant peripheral insulin resistance, hyperglycemia and moderate hyperinsulinemia. Isolated islets of HFd-fed mice displayed a significant impairment of glucose-induced insulin secretion associated with a diminished frequency of intracellular calcium oscillations compared with control islets. No marked change in islet morphology and cytoarchitecture was observeden
dc.description.abstracthowever, HFd-fed mice showed higher beta cell relative area in comparison with controls. As shown by immunohistochemistry, ZO-1, E-, N-cadherins, alpha- and beta-catenins were expressed at the intercellular contact site of endocrine cells, while VE-cadherin, as well as ZO-1, was found at islet vascular compartment. Redistribution of N-, E-cadherins and alpha-catenin (from the contact region to the cytoplasm in endocrine cells) associated with increased submembranous F-actin cell level as well as increased VE-cadherin islet immunolabeling was observed in diabetic mice. Increased gene expression of VE-cadherin and ZO-1, but no change for the other proteins, was observed in islets of diabetic mice. Only in the case of VE-cadherin, a significant increase in islet content of this CAM was detected by immunoblotting in diabetic mice. In conclusion, CAMs are expressed by endocrine and endothelial cells of pancreatic islets. The distribution/expression of N-, E- and VE-cadherins as well as alpha-catenin and F-actin is significantly altered in islet cells of obese and diabetic mice.en
dc.description.affiliationUniv Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Pernambuco UPE, Coll Nursing, Inst Biol, Recife, PE, Brazil
dc.description.affiliationFed Univ Pernambuco UFRPE, Dept Biol, Recife, PE, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biosci, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFAEPEX/UNICAMP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)/PROEX (Brazil)
dc.description.sponsorshipPhD CAPES-DINTER fellowship (Brazil)
dc.description.sponsorshipIDFAPESP: 2010/50789-1
dc.description.sponsorshipIDCNPq: 307163/2012-1
dc.description.sponsorshipIDCNPq: 304991/2015-5
dc.description.sponsorshipID2000/05137-4
dc.description.sponsorshipID2009/54129-9
dc.format.extent13-31
dc.identifierhttps://doi.org/10.1007/s00418-016-1428-5
dc.identifier.citationHistochemistry and Cell Biology. New York, v. 146, n. 1, p. 13-31, 2016.
dc.identifier.doi10.1007/s00418-016-1428-5
dc.identifier.issn0948-6143
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57685
dc.identifier.wosWOS:000378785900002
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofHistochemistry and Cell Biology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCell adhesion moleculesen
dc.subjectCell-cell interactionsen
dc.subjectPancreatic beta cellsen
dc.subjectType 2 diabetes mellitusen
dc.subjectHigh-fat dieten
dc.subjectInsulin secretionen
dc.titleReduced insulin secretion function is associated with pancreatic islet redistribution of cell adhesion molecules (CAMs) in diabetic mice after prolonged high-fat dieten
dc.typeinfo:eu-repo/semantics/article
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