Structural and biochemical alterations during the healing process of tendons treated with Aloe vera

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dc.contributor.authorAro, A. A.
dc.contributor.authorNishan, U. [UNIFESP]
dc.contributor.authorPerez, M. O.
dc.contributor.authorRodrigues, R. A.
dc.contributor.authorFoglio, M. A.
dc.contributor.authorCarvalho, J. E.
dc.contributor.authorGomes, L.
dc.contributor.authorVidal, B. C.
dc.contributor.authorPimentel, E. R.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:53Z
dc.date.available2016-01-24T14:27:53Z
dc.date.issued2012-10-29
dc.description.abstractAims: the tendon is composed of highly organized collagen fibers that form a complex supramolecular structure. After lesions, the organization and composition of the tendon are not completely restored. Our purpose was to evaluate if the application of Aloe vera improves tendon healing, considering the effectiveness in the stimulus of collagen synthesis.Main methods: the calcaneal tendon of male Wistar rats was partially transected with subsequent topical application of A. vera ointment at the injury. the animals were separated into groups with tendons treated with the A. vera extract for 7 days and excised on the 7th, 14th and 21st days after surgery: control rats received only ointment base without plant extract.Key findings: Morphological analysis using polarization microscopy showed that the entire tendon undergoes a remodeling process, with disorganized collagen fibers by days 7 and 14 in plant treated and non-treated groups and with a higher birefringence in tendons of the plant-treated group on the 21st day. A higher concentration of hydroxyproline was found in plant-treated tendons on days 7 and 14 compared with their controls. Western blots showed lower amounts of type I collagen in the plant-treated group on day 14 compared with the control. MMP-9 diminished 14 days after lesion and the active isoform of MMP-2 increased on day 21 in plant-treated groups.Significance: the present study indicates a beneficial effect of A. vera in the tissue reorganization in the transected region of the tendon 21 days after injury and is supported by an increase of active MMP-2. (C) 2012 Elsevier Inc. All rights reserved.en
dc.description.affiliationState Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationFed Univ São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, Brazil
dc.description.affiliationState Univ Campinas UNICAMP, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUnifespFed Univ São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2008/05912-0
dc.format.extent885-893
dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2012.09.002
dc.identifier.citationLife Sciences. Oxford: Pergamon-Elsevier B.V., v. 91, n. 17-18, p. 885-893, 2012.
dc.identifier.doi10.1016/j.lfs.2012.09.002
dc.identifier.issn0024-3205
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35415
dc.identifier.wosWOS:000311529300010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofLife Sciences
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTendonen
dc.subjectCollagenen
dc.subjectAloe veraen
dc.subjectHealing processen
dc.subjectRaten
dc.titleStructural and biochemical alterations during the healing process of tendons treated with Aloe veraen
dc.typeinfo:eu-repo/semantics/article
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