Paracoccidioides brasiliensis induces secretion of IL-6 and IL-8 by lung epithelial cells. Modulation of host cytokine levels by fungal proteases

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dc.contributor.authorMaza, Paloma Korehisa [UNIFESP]
dc.contributor.authorOliveira, Priscila de [UNIFESP]
dc.contributor.authorToledo, Marcos Sergio de [UNIFESP]
dc.contributor.authorDe Paula, Daisy Maria Bentes [UNIFESP]
dc.contributor.authorTakahashi, Helio Kiyoshi [UNIFESP]
dc.contributor.authorStraus, Anita Hilda [UNIFESP]
dc.contributor.authorSuzuki, Erika [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:27:44Z
dc.date.available2016-01-24T14:27:44Z
dc.date.issued2012-10-01
dc.description.abstractParacoccidioides brasiliensis is a pathogenic, dimorphic fungus that causes paracoccidioidomycosis, a systemic human mycosis that is highly prevalent in Latin America. in this study, we demonstrated that P. brasiliensis yeasts induced interleukin (IL)-8 and IL-6 secretion by human lung epithelial A549 cells. However, tumor necrosis factor-alpha and interferon-gamma were undetectable in these cultures. Moreover, P brasiliensis yeasts induced activation of p38 mitogen-activated protein kinase (MAPK), c-Jun NH2-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) 1/2 in A549 cells, and IL-8 and IL-6 secretion promoted by this fungus was dependent on activation of p38 MAPK and ERK 1/2. in addition, IL-8 and IL-6 levels were significantly higher in culture supernatants of A549 cells that were incubated with formaldehyde-fixed P brasiliensis compared to cultures of cells that were infected with live yeasts. Our results indicate that. the observed cytokine level differences were due to protease expression, in live yeasts, that degraded these cytokines. Degradation of human recombinant IL-8 and IL-6 by live P brasiliensis was inhibited by AEBSF and aprotinin, suggesting that these proteases belong to a family of serine proteases. This is the first report showing that P brasiliensis may modulate host inflammation by expressing proteases that degrade proinflammatory cytokines. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, Div Glycoconjugate Immunochem, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, Div Glycoconjugate Immunochem, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent1077-1085
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2012.05.016
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 14, n. 12, p. 1077-1085, 2012.
dc.identifier.doi10.1016/j.micinf.2012.05.016
dc.identifier.fileWOS000309441800010.pdf
dc.identifier.issn1286-4579
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35297
dc.identifier.wosWOS:000309441800010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectParacoccidioides. brasiliensisen
dc.subjectCytokinesen
dc.subjectEpithelial cellsen
dc.subjectp38 MAPKen
dc.subjectMAPK-ERK kinasesen
dc.subjectProteasesen
dc.titleParacoccidioides brasiliensis induces secretion of IL-6 and IL-8 by lung epithelial cells. Modulation of host cytokine levels by fungal proteasesen
dc.typeinfo:eu-repo/semantics/article
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