Trypanosoma cruzi persistence in the native heart is associated with high-grade myocarditis, but not with Chagas' disease reactivation after heart transplantation

Página do item simplificado
dc.contributor.authorBenvenuti, Luiz A.
dc.contributor.authorRoggerio, Alessandra
dc.contributor.authorNishiya, Anna S. [UNIFESP]
dc.contributor.authorCampos, Silvia V.
dc.contributor.authorFiorelli, Alfredo I.
dc.contributor.authorLevi, Jose E. [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:37:32Z
dc.date.available2016-01-24T14:37:32Z
dc.date.issued2014-07-01
dc.description.abstractBACKGROUND: Chagas' disease reactivation (CDR) after heart transplantation (HTx) is characterized by relapse of the infectious disease, with direct detection of Trypanosoma cruzi parasites in blood, cerebrospinal fluid, or tissues. We investigated whether a detailed pathologic examination of the explanted heart at HTx with evaluation of myocarditis and parasitic persistence or load in the myocardium could be useful to identify patients at high risk of CDR.METHODS: the native hearts of 18 chagasic patients who presented CDR after HTx (CDR+ group) were compared with the native hearts of 16 chagasic patients who never presented CDR in a follow-up of at least 18 months after HTx (CDR- group). the intensity of myocarditis was evaluated semiquantitatively. Parasite persistence/load in the myocardium was investigated through immunohistochemistry for T cruzi antigens and by qualitative and quantitative real-time PCR for T cruzi DNA.RESULTS: the rate of high-grade myocarditis, parasite persistence, and the median of parasitic load and parasitic load/10(6) cells in the CDR+ group were 83.3%, 77.8%, 8.43 x 10(-3), and 9.890, respectively, whereas in the CDR- group the values were 87.5%, 50%, 7.49 x 10(-3), and 17.800. There was no statistical difference between the groups. High-grade myocarditis was present in all 22 samples (100%) with parasite persistence and in 7 of 12 samples (58.3%) with no parasite persistence (p = 0.003).CONCLUSIONS: Although associated with high-grade myocarditis, T cruzi parasite persistence in the myocardium of the native heart is not associated with the occurrence of CDR after HTx. (C) 2014 International Society for Heart and Lung Transplantation. All rights reserved.en
dc.description.affiliationUniv São Paulo, Sch Med, Inst Heart, BR-05403000 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Fdn Pro Sangue, Hemocentro São Paulo, Dept Biol Mol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Infect Dis DIPA, Silo Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fdn Pro Sangue, Hemocentro São Paulo, Dept Biol Mol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Infect Dis DIPA, Silo Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 11/51580-1
dc.format.extent698-703
dc.identifierhttp://dx.doi.org/10.1016/j.healun.2014.01.920
dc.identifier.citationJournal of Heart and Lung Transplantation. New York: Elsevier B.V., v. 33, n. 7, p. 698-703, 2014.
dc.identifier.doi10.1016/j.healun.2014.01.920
dc.identifier.issn1053-2498
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37938
dc.identifier.wosWOS:000339532100005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Heart and Lung Transplantation
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectChagas' diseaseen
dc.subjectTrypanosoma cruzien
dc.subjectheart transplantationen
dc.subjectmyocarditisen
dc.subjectpolymerase chain reactionen
dc.titleTrypanosoma cruzi persistence in the native heart is associated with high-grade myocarditis, but not with Chagas' disease reactivation after heart transplantationen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral