Characterization of glycosaminoglycans in tubular epithelial cells: Calcium oxalate and oxalate ions effects

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dc.contributor.authorBorges, Fernanda Teixeira [UNIFESP]
dc.contributor.authorMichelacci, Yara Maria [UNIFESP]
dc.contributor.authorAguiar, Jair Adriano Kopke [UNIFESP]
dc.contributor.authorDalboni, Maria Aparecida [UNIFESP]
dc.contributor.authorGarofalo, Andrezza S.
dc.contributor.authorSchor, Nestor [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:38:04Z
dc.date.available2016-01-24T12:38:04Z
dc.date.issued2005-10-01
dc.description.abstractBackground. the interaction between tubular epithelial cells and calcium oxalate crystals or oxalate ions is a very precarious event in the lithogenesis. Urine contains ions, glycoproteins and glycosaminoglycans that inhibit the crystallization process and may protect the kidney against lithogenesis. We examined the effect of oxalate ions and calcium oxalate crystals upon the synthesis of glycosaminoglycans in distal [Madin-Darby canine kidney (MDCK)] and proximal (LLC-PK1) tubular cell lines.Methods. Glycosaminoglycan synthesis was analyzed by metabolic labeling with S-35-sulfate and enzymatic digestion with specific mucopolysaccharidases. Cell death was assessed by fluorescent dyes and crystal endocytosis was analised by flow cytometry.Results. the main glycosaminoglycans synthesized by both cells were chondroitin sulfate and heparan sulfate most of them secreted to the culture medium or present at cellular surface. Exposition of MDCK cells to oxalate ions increased apoptosis rate and the incorporation of S-35-sulfate in chondroitin sulfate and heparan sulfate, while calcium oxalate crystals were endocyted by LLC-PK1, induced necrotic cell death, and increased S-35-sulfate incorporation in glycosaminoglycans. These effects seem to be specific and due to increased biosynthesis, since hydroxyapatite and other carboxylic acid did not induced cellular death or glycosaminoglycan synthesis and no changes in sulfation degree or molecular weight of glycosaminoglycans could be detected. Thapsigargin inhibited the glycosaminoglycan synthesis induced by calcium oxalate in LLC-PK1, suggesting that this effect was sensitive to the increase in cytosolic calcium.Conclusion. Tubular cells may increase the synthesis of glycosaminoglycans to protect from the toxic insult of calcium oxalate crystals and oxalate ions, what could partially limit the lithogenesis.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, Disciplina Nefrol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, Disciplina Biol Mol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, Disciplina Nefrol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, Disciplina Biol Mol, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent1630-1642
dc.identifierhttp://dx.doi.org/10.1111/j.1523-1755.2005.00577.x
dc.identifier.citationKidney International. Oxford: Blackwell Publishing, v. 68, n. 4, p. 1630-1642, 2005.
dc.identifier.doi10.1111/j.1523-1755.2005.00577.x
dc.identifier.issn0085-2538
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28479
dc.identifier.wosWOS:000231801300028
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofKidney International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcalcium oxalateen
dc.subjectpotassium oxalateen
dc.subjectglycosaminoglycanen
dc.subjectproteoglycanen
dc.subjectMDCKen
dc.subjectLLC-PK1en
dc.titleCharacterization of glycosaminoglycans in tubular epithelial cells: Calcium oxalate and oxalate ions effectsen
dc.typeinfo:eu-repo/semantics/article
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