Altered reactivity of gastric fundus smooth muscle in the mouse with targeted disruption of the kinin B(1) receptor gene

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dc.contributor.authorBarbosa, Ana M. R. B. [UNIFESP]
dc.contributor.authorFelipe, Sandra A. [UNIFESP]
dc.contributor.authorAraujo, Ronaldo C. [UNIFESP]
dc.contributor.authorKawamoto, Elisa M.
dc.contributor.authorCarvalho, Maria H. C.
dc.contributor.authorScavone, Cristoforo
dc.contributor.authorPesquero, Joao B. [UNIFESP]
dc.contributor.authorShimuta, Suma I. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T13:52:30Z
dc.date.available2016-01-24T13:52:30Z
dc.date.issued2009-05-01
dc.description.abstractRelaxing action of sodium nitroprusside (SNP) was significantly reduced in the stomach fundus of mice lacking the kinin B(1) receptor (B(1)(-/-)). Increased basal cGMP accumulation was correlated with attenuated SNP induced dose-dependent relaxation in B(1)(-/-) when compared with wild type (WT) control mice. These responses to SNP were completely blocked by the guanylate cyclase inhibitor ODQ(10 mu M). It was also found that Ca(2+)-dependent, constitutive nitric oxide synthase (cNOS) activity was unchanged but the Ca(2+)-independent inducible NOS (iNOS) activity was greater in B(1)(-/-) mice than in WT animals. Zaprinast (100 mu M), a specific phosphodiesterase inhibitor, increased the nitrergic relaxations and the accumulation of the basal as well as the SNP-stimulated cGMP in WT but not in B(1)(-/-) stomach fundus. From these findings it is concluded that the inhibited phosphodiesterase activity and high level of cGMP reduced the resting muscle tone, impairing the relaxant responses of the stomach in B(1)(-/-) mice. in addition, it can be suggested that functional B(2) receptor might be involved in the NO compensatory mechanism associated with the deficiency of kinin B(1) receptor in the gastric tissue of the transgenic mice. (C) 2009 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista São Paulo, Dept Biophys, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, BR-05508 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista São Paulo, Dept Biophys, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent901-905
dc.identifierhttp://dx.doi.org/10.1016/j.peptides.2009.01.025
dc.identifier.citationPeptides. New York: Elsevier B.V., v. 30, n. 5, p. 901-905, 2009.
dc.identifier.doi10.1016/j.peptides.2009.01.025
dc.identifier.issn0196-9781
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31501
dc.identifier.wosWOS:000266369900011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPeptides
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectKinin B(1) receptor knockout mouseen
dc.subjectRelaxant responsesen
dc.subjectMouse gastric fundusen
dc.subjectcGMPen
dc.subjectPhosphodiesteraseen
dc.titleAltered reactivity of gastric fundus smooth muscle in the mouse with targeted disruption of the kinin B(1) receptor geneen
dc.typeinfo:eu-repo/semantics/article
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