Increased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp massiliense CRM0019 in alveolar epithelial cells

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dc.citation.volume17
dc.contributor.authorRibeiro, Giovanni Monteiro [UNIFESP]
dc.contributor.authorMatsumoto, Cristianne Kayoko [UNIFESP]
dc.contributor.authorReal, Fernando [UNIFESP]
dc.contributor.authorTeixeira, Daniela [UNIFESP]
dc.contributor.authorDuarte, Rafael Silva
dc.contributor.authorMortara, Renato Arruda [UNIFESP]
dc.contributor.authorLeao, Sylvia Cardoso [UNIFESP]
dc.contributor.authorCarvalho-Wodarz, Cristiane de Souza [UNIFESP]
dc.coverageLondon
dc.date.accessioned2020-08-04T13:40:12Z
dc.date.available2020-08-04T13:40:12Z
dc.date.issued2017
dc.description.abstractBackground: Outbreaks of infections caused by rapidly growing mycobacteria have been reported worldwide generally associated with medical procedures. Mycobacterium abscessus subsp. massiliense CRM0019 was obtained during an epidemic of postsurgical infections and was characterized by increased persistence in vivo. To better understand the successful survival strategies of this microorganism, we evaluated its infectivity and proliferation in macrophages (RAW and BMDM) and alveolar epithelial cells (A549). For that, we assessed the following parameters, for both M. abscessus CRM0019 as well as the reference strain M. abscessus ATCC 19977: internalization, intracellular survival for up 3 days, competence to subvert lysosome fusion and the intracellular survival after cell reinfection. Results: CRM0019 and ATCC 19977 strains showed the same internalization rate (approximately 30% after 6 h infection), in both A549 and RAW cells. However, colony forming units data showed that CRM0019 survived better in A549 cells than the ATCC 19977 strain. Phagosomal characteristics of CRM0019 showed the bacteria inside tight phagosomes in A549 cells, contrasting to the loosely phagosomal membrane in macrophages. This observation holds for the ATCC 19977 strain in both cell types. The competence to subvert lysosome fusion was assessed by acidification and acquisition of lysosomal protein. For M. abscessus strains the phagosomes were acidified in all cell linesen
dc.description.abstractnevertheless, the acquisition of lysosomal protein was reduced by CRM0019 compared to the ATCC 19977 strain, in A549 cells. Conversely, in macrophages, both M. abscessus strains were located in mature phagosomes, however without bacterial death. Once recovered from macrophages M. abscessus could establish a new intracellular infection. Nevertheless, only CRM0019 showed a higher growth rate in A549, increasing nearly 10- fold after 48 and 72 h. Conclusion: M. abscessus CRM0019 creates a protective and replicative niche in alveolar epithelial cells mainly by avoiding phagosome maturation. Once recovered from infected macrophages, CRM0019 remains infective and displays greater intracellular growth in A549 cells compared to the ATCC 19977 strain. This evasion strategy in alveolar epithelial cells may contribute to the long survival of the CRM0019 strain in the host and thus to the inefficacy of in vivo treatment.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, SP, Brazil
dc.description.affiliationInst Cochin, Dept Infect Immunite & Inflammat, Lab Entree Muqueuse VIH & Immunite Muqueuse, Paris, France
dc.description.affiliationInst Microbiol Prof Paulo de Goes, Lab Micobacterias, Cidade Univ, Rio De Janeiro, Brazil
dc.description.affiliationHelmholtz Ctr Infect Res HZI, Helmholtz Inst Pharmaceut Res Saarland HIPS, Dept Drug Delivery, Saarbrucken, Germany
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP)
dc.description.sponsorshipIDFAPESP: 2012/04913-8
dc.description.sponsorshipIDFAPESP: 2013/16018-6
dc.description.sponsorshipIDFAPESP: 2009/14665-9
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1186/s12866-017-1102-7
dc.identifier.citationBmc Microbiology. London, v. 17, p. -, 2017.
dc.identifier.doi10.1186/s12866-017-1102-7
dc.identifier.fileWOS000410951100002.pdf
dc.identifier.issn1471-2180
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57360
dc.identifier.wosWOS:000410951100002
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMycobacterium abscessusen
dc.subjectPhagosomeen
dc.subjectAcidificationen
dc.subjectA549en
dc.subjectMacrophagesen
dc.subjectCRM0019en
dc.titleIncreased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp massiliense CRM0019 in alveolar epithelial cellsen
dc.typeinfo:eu-repo/semantics/article
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