Xanthomonas citri subsp citri surface proteome by 2D-DIGE: Ferric enterobactin receptor and other outer membrane proteins potentially involved in citric host interaction

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dc.citation.volume151
dc.contributor.authorCarnielli, Carolina Moretto
dc.contributor.authorArtier, Juliana
dc.contributor.authorFranco de Oliveira, Julio Cezar [UNIFESP]
dc.contributor.authorMarques Novo-Mansur, Maria Teresa
dc.coverageAmsterdam
dc.date.accessioned2020-07-17T14:03:14Z
dc.date.available2020-07-17T14:03:14Z
dc.date.issued2017
dc.description.abstractXanthomonas citri subsp. citri (XAC) is the causative agent of citrus canker, a disease of great economic impact around the world. Understanding the role of proteins on XAC cellular surface can provide new insights on pathogen-plant interaction. Surface proteome was performed in XAC grown in vivo (infectious) and in vitro (non-infectious) conditions, by labeling intact cells followed by cellular lysis and direct 2D-DIGE analysis. Seventy-nine differential spots were analyzed by mass spectrometry. Highest relative abundance for in vivo condition was observed for spots containing Dnal(protein, 60 kDa chaperonin, conserved hypothetical proteins, malate dehydrogenase, phosphomannose isomerase, and ferric enterobactin receptors. Elongation factor Tu, OmpA-related proteins, Oar proteins and some Ton-B dependent receptors were found in spots decreased in vivo. Some proteins identified on XAC's surface in infectious condition and predicted to be cytoplasmic, such as DnaK and 60 KDa chaperonin, have also been previously found at cellular surface in other microorganisms. This is the first study on XAC surface proteome and results point to mediation of molecular chaperones in XAC-citrus interaction. The approach utilized here can be applied to other pathogen-host interaction systems and help to achieve new insights in bacterial pathogenicity toward promising targets of biotechnological interest. Biological significance: This research provides new insights for current knowledge of the Xanthomonas sp. pathogenicity. For the first time the 2D-DIGE approach was applied on intact cells to find surface proteins involved in the pathogen-plant interaction. Results point to the involvement of new surface/outer membrane proteins in the interaction between XAC and its citrus host and can provide potential targets of biotechnological interest for citrus canker control. (C) 2016 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Sao Carlos, UFSCar, Dept Genet & Evolucao, Lab Bioquim & Biol Mol Aplicada, Sao Carlos, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ciencias Biol, Lab Interacoes Microbianas, UNIFESP, Diadema, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ciencias Biol, Lab Interacoes Microbianas, UNIFESP, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipMass Spectrometry Laboratory at Brazilian Biosciences Nacional Laboratory (LNBio), CNPEM, Campinas-SP, Brazil
dc.description.sponsorshipIDFAPESP: 07/50910-2
dc.description.sponsorshipIDCNPq: 132940/2011-6
dc.format.extent251-263
dc.identifierhttp://dx.doi.org/10.1016/j.jprot.2016.05.007
dc.identifier.citationJournal Of Proteomics. Amsterdam, v. 151, p. 251-263, 2017.
dc.identifier.doi10.1016/j.jprot.2016.05.007
dc.identifier.issn1874-3919
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/55241
dc.identifier.wosWOS:000390964900025
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofJournal Of Proteomics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCitrus cankeren
dc.subjectXanthomonas citri subsp citrien
dc.subjectPathogenicityen
dc.subject2D-DIGEen
dc.subjectSurface proteomeen
dc.subjectChaperoneen
dc.titleXanthomonas citri subsp citri surface proteome by 2D-DIGE: Ferric enterobactin receptor and other outer membrane proteins potentially involved in citric host interactionen
dc.typeinfo:eu-repo/semantics/article
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