Cyclooxygenase-2 and vascular endothelial growth factor expression in 5-fluorouracil-induced oral mucositis in hamsters: evaluation of two low-intensity laser protocols

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dc.contributor.authorFontana Lopes, Nilza Nelly [UNIFESP]
dc.contributor.authorPlapler, Helio [UNIFESP]
dc.contributor.authorChavantes, Maria Cristina
dc.contributor.authorLalla, Rajesh V.
dc.contributor.authorYoshimura, Elisabeth Mateus
dc.contributor.authorSeixas Alves, Maria Teresa [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Connecticut
dc.date.accessioned2016-01-24T13:58:52Z
dc.date.available2016-01-24T13:58:52Z
dc.date.issued2009-11-01
dc.description.abstractThe aim of this study was to investigate the mechanisms whereby low-intensity laser therapy may affect the severity of oral mucositis.A hamster cheek pouch model of oral mucositis was used with all animals receiving intraperitoneal 5-fluorouracil followed by surface irritation. Animals were randomly allocated into three groups and treated with a 35 mW laser, 100 mW laser, or no laser. Clinical severity of mucositis was assessed at four time-points by a blinded examiner. Buccal pouch tissue was harvested from a subgroup of animals in each group at four time-points. This tissue was used for immunohistochemistry for cyclooxygenase-2 (COX-2), vascular endothelial growth factor (VEGF), and factor VIII (marker of microvessel density) and the resulting staining was quantified.Peak severity of mucositis was reduced in the 35 mW laser group as compared to the 100 mW laser and control groups. This reduced peak clinical severity of mucositis in the 35 mW laser group was accompanied by a significantly lower level of COX-2 staining. the 100 mW laser did not have an effect on the severity of clinical mucositis, but was associated with a decrease in VEGF levels at the later time-points, as compared to the other groups. There was no clear relationship of VEGF levels or microvessel density to clinical mucositis severity.The tissue response to laser therapy appears to vary by dose. Low-intensity laser therapy appears to reduce the severity of mucositis, at least in part, by reducing COX-2 levels and associated inhibition of the inflammatory response.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Expt Surg, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Med Laser Ctr, INCOR HC, BR-05403000 São Paulo, Brazil
dc.description.affiliationUniv Connecticut, Ctr Hlth, Sect Oral Med, Dept Oral Hlth & Diagnost Sci MC1605, Farmington, CT 06030 USA
dc.description.affiliationUniv São Paulo, Inst Phys, Dept Nucl Phys, BR-05315970 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pathol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Expt Surg, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pathol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1409-1415
dc.identifierhttp://dx.doi.org/10.1007/s00520-009-0603-9
dc.identifier.citationSupportive Care in Cancer. New York: Springer, v. 17, n. 11, p. 1409-1415, 2009.
dc.identifier.doi10.1007/s00520-009-0603-9
dc.identifier.issn0941-4355
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31916
dc.identifier.wosWOS:000270842000009
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofSupportive Care in Cancer
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectLow-intensity laseren
dc.subjectChemotherapyen
dc.subjectCyclooxygenase-2en
dc.subjectVascular endothelial growth factoren
dc.subjectOral mucositisen
dc.titleCyclooxygenase-2 and vascular endothelial growth factor expression in 5-fluorouracil-induced oral mucositis in hamsters: evaluation of two low-intensity laser protocolsen
dc.typeinfo:eu-repo/semantics/article
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