Plasma-kallikrein clearance during liver regeneration after partial hepatectomy in the rat

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dc.contributor.authorBraulio, Valeria Bender
dc.contributor.authorKouyoumdjian, Maria [UNIFESP]
dc.contributor.authorZucoloto, Sergio
dc.contributor.authorFigueiredo, Florencio
dc.contributor.authorBorges, Durval Rosa [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-06-18T11:03:56Z
dc.date.available2018-06-18T11:03:56Z
dc.date.issued1998-12-01
dc.description.abstractAims/Background. The liver clears circulating plasma-kallikrein through a receptor-mediated endocytosis process: an initial fast phase is followed by a slow exponential phase. Methods: To determine whether the clearance rate of plasma-kallikrein is affected during liver regeneration, we perfused isolated rat livers with rat plasma-kallikrein (rPK) at 0, 1, 2, 3 and 7 days after partial hepatectomy or sham operation. Results. Liver regeneration was followed by the expression of the proliferating-cell nuclear antigen (PCNA) labeling index. The serum concentration of alpha(2)-macroglobulin, an acute phase protein in rats, was measured. At day 1, the fast phase of rPK clearance rate increased in hepatectomized rats when compared with day 0 (4.9+/-0.4 and 3.7+/-0.4 mU/g liver min, p<0.05). However, at day 2, the rPK fast phase clearance rate dropped significantly (2.6+/-0.2, p<0.05), when compared with day 1. No difference was found among the sham groups at different days of hepatectomy. These changes seem to be independent of the acute phase reaction. The regenerative liver weight increased continuously during the observation period. PCNA expression increased significantly after hepatectomy, with maximal PCNA-labeling indices at days 1 and 2, declining thereafter. Conclusion. The rPK fast phase clearance rate changes during liver regeneration, with a zenith occurring when PCNA labeling index is maximal (day 1) and a nadir occurring at the mitotic phase (day 2).en
dc.description.affiliationUniv Fed Sao Paulo, Lab Hepatol Expt, BR-04034970 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Dept Med, BR-21941 Rio De Janeiro, Brazil
dc.description.affiliationUniv Sao Paulo, Med Sch Ribeirao Preto, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Lab Hepatol Expt, BR-04034970 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent371-377
dc.identifierhttp://dx.doi.org/10.1111/j.1600-0676.1998.tb00820.x
dc.identifier.citationLiver. Copenhagen: Munksgaard Int Publ Ltd, v. 18, n. 6, p. 371-377, 1998.
dc.identifier.doi10.1111/j.1600-0676.1998.tb00820.x
dc.identifier.issn0106-9543
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44860
dc.identifier.wosWOS:000077455600001
dc.language.isoeng
dc.publisherMunksgaard Int Publ Ltd
dc.relation.ispartofLiver
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectliver clearanceen
dc.subjectliver regenerationen
dc.subjectpartial hepatectomyen
dc.subjectplasma-kallikreinen
dc.titlePlasma-kallikrein clearance during liver regeneration after partial hepatectomy in the raten
dc.typeinfo:eu-repo/semantics/article
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