Enzyme and integrin expression by high and low metastatic melanoma cell lines

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dc.contributor.authorStaquicini, F. I.
dc.contributor.authorMoreira, C. R.
dc.contributor.authorNascimento, F. D.
dc.contributor.authorTersariol, ILS
dc.contributor.authorNader, H. B.
dc.contributor.authorDietrich, C. P.
dc.contributor.authorLopes, J. D.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Mogi Cruzes
dc.date.accessioned2016-01-24T12:33:43Z
dc.date.available2016-01-24T12:33:43Z
dc.date.issued2003-02-01
dc.description.abstractDissemination of a malignant tumour is the result of a cascade of events beginning with detachment of cells from primary tumour followed by extravasation and growth at secondary sites. the differences in metastatic ability could be attributed to properties intrinsic to the various tumour types. Thus the clonal selection of tumour cells from successive metastases apparently results in cells better equipped for survival and formation of colonies in secondary sites, indicating that survival is not a random phenomenon. Many studies of malignant cells have correlated the overexpression of adhesion receptors such as integrins and the production of cysteine proteases and glycosidases with the progression of malignancy. the interaction of cysteine proteases with basement membrane components has been implicated in tumour invasion, activation of hormones and growth factors. On the other hand, the expression of the heparanase gene and its protein has been associated with the metastatic potential of several human and mouse tumour cell lines. This study aimed to investigate the correlations between the metastatic properties of clones with high and low metastatic potential and their ability to adhere to the extracellular matrix and to degrade proteins and sulphated glycosaminoglycans present there. Clonal selection of the B16F10 cell line was performed, and the clones were examined for the expression of an integrin-type laminin receptor. A significantly higher level was detected in a high metastatic clone. Enzymatic assays showed higher activity for alpha-D-N-acetylglucosaminidase, beta-D-N-acetylgalactosaminidase and beta-D-glucuronidase in conditioned medium from low metastatic clones compared with that from high metastatic clones. However, higher endopeptidase activity was observed in conditioned medium from high metastatic clones. in summary, these results showed a positive correlation between high metastatic potential and endopeptidase secretion. Similarly, a positive correlation was observed between low metastatic cells and the secretion of glycosaminoglycan-degrading glycosidases.en
dc.description.affiliationUniversidade Federal de São Paulo, Disciplina Imunol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Mol Biol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Mogi Cruzes, Interdisciplinary Ctr Biochem Invest, Mogi Das Cruzes, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Disciplina Imunol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Mol Biol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent11-18
dc.identifierhttp://dx.doi.org/10.1097/01.cmr.0000056208.78713.6f
dc.identifier.citationMelanoma Research. Philadelphia: Lippincott Williams & Wilkins, v. 13, n. 1, p. 11-18, 2003.
dc.identifier.doi10.1097/01.cmr.0000056208.78713.6f
dc.identifier.issn0960-8931
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27146
dc.identifier.wosWOS:000181385500003
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofMelanoma Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectmetastasesen
dc.subjectintegrinen
dc.subjectenzymeen
dc.subjectmelanomaen
dc.subjectproteoglycanen
dc.subjectglycosaminoglycanen
dc.titleEnzyme and integrin expression by high and low metastatic melanoma cell linesen
dc.typeinfo:eu-repo/semantics/article
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