Leptin Downregulates LPS-Induced Lung Injury: Role of Corticosterone and Insulin

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dc.contributor.authorLandgraf, Maristella de Almeida [UNIFESP]
dc.contributor.authorSilva, Reinaldo Correia [UNIFESP]
dc.contributor.authorCorrea-Costa, Matheus
dc.contributor.authorHiyane, Meire Ioshie [UNIFESP]
dc.contributor.authorCarvalho, Maria Helena C.
dc.contributor.authorLandgraf, Richardt Gama [UNIFESP]
dc.contributor.authorCâmara, Niels Olsen Saraiva [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:35:01Z
dc.date.available2016-01-24T14:35:01Z
dc.date.issued2014-01-01
dc.description.abstractBackground/Aims: We investigated the effects of leptin in the development of lipopolysaccharide (LPS)-induced acute lung inflammation (ALI) in lean mice. Methods: Mice were administered leptin (1.0 mu g/g) or leptin (1.0 mu g/g) followed by LPS (1.5 mu g/g) intranasally. Additionally, some animals were given LPS (1.5 mu g/g) or saline intranasally alone, as a control. Tissue samples and fluids were collected six hours after instillation. Results: We demonstrated that leptin alone did not induce any injury. Local LPS exposure resulted in significant acute lung inflammation, characterized by a substantial increase in total cells, mainly neutrophils, in bronchoalveolar lavages (BAL). We also observed a significant lymphocyte influx into the lungs associated with enhanced lung expression of chemokines and cytokines (KC, RANTES, TNF-alpha, IFN-beta, GM-CSF and VEGF). LPS-induced ALI was characterized by the enhanced expression of ICAM-1 and iNOS in the lungs. Mice that received LPS showed an increase in insulin levels. Leptin, when administered prior to LPS instillation, abolished all of these effects. LPS induced an increase in corticosterone levels, and leptin potentiated this event. Conclusion: These data suggest that exogenous leptin may promote protection during sepsis, and downregulation of the insulin levels and upregulation of corticosterone may be important mechanisms in the amelioration of LPS-induced ALI.Copyright (c) 2014 S. Karger AG, Baselen
dc.description.affiliationUniv São Paulo, Inst Biomed Sci 1, Dept Pharmacol, Lab Hypertens, BR-1524 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Immunol, Lab Transplantat Immunobiol, BR-1524 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Lab Inflammat & Vasc Pharmacol, BR-05508 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Nephrol, Lab Clin & Expt Immunobiol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nephrol, Lab Clin & Expt Immunobiol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipComplex Fluids INCT
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 12/51104-8
dc.description.sponsorshipIDFAPESP: 10/01404-0
dc.description.sponsorshipIDFAPESP: 12/02270-2
dc.description.sponsorshipIDFAPESP: 12/10512-6
dc.format.extent835-846
dc.identifierhttp://dx.doi.org/10.1159/000358656
dc.identifier.citationCellular Physiology and Biochemistry. Basel: Karger, v. 33, n. 3, p. 835-846, 2014.
dc.identifier.doi10.1159/000358656
dc.identifier.fileWOS000334493600024.pdf
dc.identifier.issn1015-8987
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37209
dc.identifier.wosWOS:000334493600024
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofCellular Physiology and Biochemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjectLeptinen
dc.subjectInsulin, corticosterone, acute lung injuryen
dc.subjectLipopolysaccharideen
dc.subjectLung inflammationen
dc.titleLeptin Downregulates LPS-Induced Lung Injury: Role of Corticosterone and Insulinen
dc.typeinfo:eu-repo/semantics/article
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