Effects of acute aerobic exercise on leukocyte inflammatory gene expression in systemic lupus erythematosus

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dc.contributor.authorPerandini, L. A.
dc.contributor.authorSales-de-Oliveira, Diego
dc.contributor.authorAlmeida, D. C. [UNIFESP]
dc.contributor.authorAzevedo, Hatylas
dc.contributor.authorMoreira-Filho, C. A.
dc.contributor.authorCenedeze, Marco Antonio [UNIFESP]
dc.contributor.authorBenatti, F. B.
dc.contributor.authorLima, F. R.
dc.contributor.authorBorba, Eduardo
dc.contributor.authorBonfa, E.
dc.contributor.authorSa-Pinto, A. L.
dc.contributor.authorRoschel, H.
dc.contributor.authorCamara, N. O.
dc.contributor.authorGualano, B.
dc.date.accessioned2019-01-21T10:29:44Z
dc.date.available2019-01-21T10:29:44Z
dc.date.issued2016
dc.description.abstractSystemic lupus erythematosus (SLE) is an autoimmune disease with a persistent systemic inflammation. Exercise-induced inflammatory response in SLE remains to be fully elucidated. The aim of this study was to assess the effects of acute exercise on leukocyte gene expression in active (SLEACTIVE) and inactive SLE (SLEINACTIVE) patients and healthy controls (HC). Methods: All subjects (n = 4 per group) performed a 30-min single bout of acute aerobic exercise (similar to 70% of VO2 peak) on a treadmill, and blood samples were collected for RNA extraction from circulating leukocyte at baseline, at the end of exercise, and after three hours of recovery. The expression of a panel of immune-related genes was evaluated by a quantitative PCR array assay. Moreover, network-based analyses were performed to interpret transcriptional changes occurring after the exercise challenge. Results: In all groups, a single bout of acute exercise led to the down-regulation of the gene expression of innate and adaptive immunity at the end of exercise (e.g., TLR3, IFNG, GATA3, FOXP3, STAT4) with a subsequent up-regulation occurring upon recovery. Exercise regulated the expression of inflammatory genes in the blood leukocytes of the SLE patients and HC, although the SLE groups exhibited fewer modulated genes and less densely connected networks (number of nodes: 29, 40 and 58en
dc.description.abstractnumber of edges: 29, 60 and 195en
dc.description.abstractnetwork density: 0.07, 0.08 and 0.12, for SLEACTIVE, SLEINACTIVE and HC, respectively). Conclusion: The leukocytes from the SLE patients, irrespective of disease activity, showed a down-regulated inflammatory gene expression immediately after acute aerobic exercise, followed by an up-regulation at recovery. Furthermore, less organized gene networks were observed in the SLE patients, suggesting that they may be deficient in triggering a normal exercise-induced immune transcriptional response.en
dc.description.affiliationRheumatology Division, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationLaboratory of Clinical and Experimental Immunology, Department of Medicine, Division of Nephrology, Federal University of Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationLaboratory of Pediatric Genomics, Department of Pediatrics, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationLaboratory of Transplantation Immunobiology, Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationSchool of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil
dc.description.affiliationUnifespLaboratory of Clinical and Experimental Immunology, Department of Medicine, Division of Nephrology, Federal University of Sao Paulo, Sao Paulo, Brazil.
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.format.extent64-80
dc.identifierhttp://eir-isei.de/2016/eir-2016-064-article.pdf
dc.identifier.citationExercise Immunology Review. Greven, v. 22, p. 64-80, 2016.
dc.identifier.issn1077-5552
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49360
dc.identifier.wosWOS:000387132000005
dc.language.isoeng
dc.publisherCentro Federal Educacao Tecnologica Rio Grande Norte
dc.relation.ispartofExercise Immunology Review
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPhysical Activityen
dc.subjectInflammationen
dc.subjectAutoimmunityen
dc.subjectGene Arrayen
dc.subjectExercise Immunologyen
dc.subjectNetwork Analysisen
dc.subjectHubsBlood Mononuclear-Cellsen
dc.subjectAutoimmune Rheumatic-Diseasesen
dc.subjectImmune-Responseen
dc.subjectTnf-Alphaen
dc.subjectAccelerated Atherosclerosisen
dc.subjectMicrorna Expressionen
dc.subjectMultiple-Sclerosisen
dc.subjectPhysical-Activityen
dc.subjectHeart-Failureen
dc.subjectMessenger-Rnaen
dc.titleEffects of acute aerobic exercise on leukocyte inflammatory gene expression in systemic lupus erythematosusen
dc.typeinfo:eu-repo/semantics/review
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