Characterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collection

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dc.contributor.authorZanetti, C.c.s.
dc.contributor.authorMingrone, R.c.c.
dc.contributor.authorKisielius, J.j.
dc.contributor.authorUeda-ito, M.
dc.contributor.authorPignatari, Antonio Carlos Campos [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionInstituto Adolfo Lutz Nucleo de Microscopia Eletronica
dc.date.accessioned2015-06-14T13:45:35Z
dc.date.available2015-06-14T13:45:35Z
dc.date.issued2013-08-13
dc.description.abstractSome clinical isolates of Pseudomonas aeruginosa stored in our culture collection did not grow or grew poorly and showed lysis on the culture plates when removed from the collection and inoculated on MacConkey agar. One hypothesis was that bacteriophages had infected and killed those clinical isolates. To check the best storage conditions to maintain viable P. aeruginosa for a longer time, clinical isolates were stored at various temperatures and were grown monthly. We investigated the presence of phage in 10 clinical isolates of P. aeruginosa stored in our culture collection. Four strains of P. aeruginosa were infected by phages that were characterized by electron microscopy and isolated to assess their ability to infect. The best condition to maintain the viability of the strains during storage was in water at room temperature. Three Siphoviridae and two Myoviridae phages were visualized and characterized by morphology. We confirmed the presence of bacteriophages infecting clinical isolates, and their ability to infect and lyse alternative hosts. Strain PAO1, however, did not show lysis to any phage. Mucoid and multidrug resistant strains of P. aeruginosa showed lysis to 50% of the phages tested.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Divisao de Doencas Infecciosas Laboratorio Especial de Microbiologia Clinica
dc.description.affiliationInstituto Adolfo Lutz Nucleo de Microscopia Eletronica
dc.description.affiliationUnifespUNIFESP, Divisao de Doencas Infecciosas Laboratorio Especial de Microbiologia Clinica
dc.description.sourceSciELO
dc.format.extent689-695
dc.identifierhttp://dx.doi.org/10.1590/1414-431X20132796
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 46, n. 8, p. 689-695, 2013.
dc.identifier.doi10.1590/1414-431X20132796
dc.identifier.fileS0100-879X2013000800689.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2013000800689
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/7911
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBacteriophagesen
dc.subjectPseudomonas aeruginosaen
dc.titleCharacterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collectionen
dc.typeinfo:eu-repo/semantics/article
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