The antiviral effect of mollusk mucus on measles virus

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dc.citation.volume134
dc.contributor.authorde Toledo-Piza, Ana Rita
dc.contributor.authorFigueiredo, Cristina Adelaide
dc.contributor.authorde Oliveira, Maria Isabel
dc.contributor.authorNegri, Giuseppina [UNIFESP]
dc.contributor.authorNamiyama, Gislene
dc.contributor.authorTonelotto, Mariana
dc.contributor.authorVillar, Karina de Senna
dc.contributor.authorRofatto, Henrique Krambeck
dc.contributor.authorMendonca, Ronaldo Zucatelli
dc.coverageAmsterdam
dc.date.accessioned2020-07-31T12:47:39Z
dc.date.available2020-07-31T12:47:39Z
dc.date.issued2016
dc.description.abstractMeasles is a viral disease highly contagious spread by respiratory transmission. Although infection can be controlled by vaccination, numerous cases of measles have been registered in many areas of the world, highlighting the need for additional interventions. Terrestrial gastropods exude mucus on their body surface when traveling, to protect the body from mechanical injury, desiccation or contact with harmful substances. The mucus of mollusks has been studied as a source of new natural compounds with diverse biological activities. In this study, the antiviral activity of the mucus of the land slug P. boraceiensis was demonstrated in vitro using Vero cells infected with measles virus. The crude sample and four fractions were tested in cultures infected with measles virus and the antiviral activity was assessed by the cytopathic effect in infected cell cultures as well as by immunofluorescence and qPCR. Fractions 39 and 50 of the mucus from P. boraceiensis were analyzed by HPLC-DAD-ESI-MS/MS and infrared spectroscopy. A mixture of polyunsaturated fatty acids was found in the two fractions. A reduction in the growth of the measles virus was observed, measured by qPCR, with a protection index of 80% in Vero cells infected with measles and treated with fraction 39. Fraction 39 exhibited the best antiviral action in vitro and high contents of hydroxy-tritriacontapentaenoic acid and hydroxy-pentatriacontapentaenoic acid were found in this fraction. (C) 2016 Elsevier B.V. All rights reserved.en
dc.description.affiliationButantan Inst, Parasitol Lab, 1500th Vital Brazil Ave, Sao Paulo, SP, Brazil
dc.description.affiliationAdolfo Lutz Inst, Resp Infect Dis, 355th Doutor Arnaldo Ave, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Prevent Med, 740th Botucatu St, Sao Paulo, SP, Brazil
dc.description.affiliationAdolfo Lutz Inst, Electron Microscopy Ctr, 355th Doutor Arnaldo Ave, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespDepartment of Preventive Medicine, Universidade Federal de São Paulo (UNIFESP), 740th, Botucatu St., São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent172-181
dc.identifierhttp://dx.doi.org/10.1016/j.antiviral.2016.09.005
dc.identifier.citationAntiviral Research. Amsterdam, v. 134, p. 172-181, 2016.
dc.identifier.doi10.1016/j.antiviral.2016.09.005
dc.identifier.issn0166-3542
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56972
dc.identifier.wosWOS:000386983800020
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofAntiviral Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAntiviralen
dc.subjectDrugsen
dc.subjectMucusen
dc.subjectMollusksen
dc.subjectLipidsen
dc.titleThe antiviral effect of mollusk mucus on measles virusen
dc.typeinfo:eu-repo/semantics/article
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