Role of interplay between IL-4 and IFN-gamma in the in regulating M1 macrophage polarization induced by Nattectin

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dc.contributor.authorIshizuka, Edson Kiyotaka
dc.contributor.authorFerreira, Marcio Jose
dc.contributor.authorGrund, Lidiane Zito
dc.contributor.authorCoutinho, Erica Maria Martins
dc.contributor.authorKomegae, Evilin Naname
dc.contributor.authorCassado, Alexandra dos Anjos [UNIFESP]
dc.contributor.authorBortoluci, Karina Ramalho [UNIFESP]
dc.contributor.authorLopes-Ferreira, Monica
dc.contributor.authorLima, Carla
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:28:03Z
dc.date.available2016-01-24T14:28:03Z
dc.date.issued2012-12-01
dc.description.abstractRecently our group described that Nattectin, a C-type lectin of the venom of Thalassophryne nattereri shows a potent pro-inflammatory capacity. Here, we demonstrated that Nattectin is able to induce M1 macrophage marker iNOS, and up-regulate the expression of MHC class II, CD80, CD86 and CD40 molecules. the increase in MHC class II and CD49a integrin expression with MMP-9 production and endocytic capacity depend on lectin function of Nattectin. Moreover, the polarization of peritoneal and bone marrow-derived macrophages induced by Nattectin to M1 profile is dependent on Th1 cytokines (IL-12 and IFN-gamma), and negatively regulated by Th2 cytokines (IL-4, IL-10 and IL-13). Also we reveal that IL-4 play a dual role in this polarization: a regular action of IL-4 was seen in the negative regulation of the CD40 expression, but an unexpected positive regulation was seen in the expression of CCR7 and MHC class II. Finally, our in vivo studies showed that the influx of neutrophils and small peritoneal macrophage - F4/80(low)MHCII(hi) induced by Nattectin is totally dependent on IL-4 and IFN-gamma cytokines. Furthermore, the induction of IL-6 release is negatively regulated by IL-4 and positively regulated by IL-12 and IFN-gamma. Together, the results allowed us to expand the knowledge about the regulation of macrophage activation, as well as confirmed the ability of Nattectin, a fish C-type lectin, as an important immunomodulatory agent. (c) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationInst Butantan, Unidade Imunorregulacao, Lab Especial Toxinol Aplicada, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Mol Immunol Innate Recognit Unit, Dept Biol Sci, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Mol Immunol Innate Recognit Unit, Dept Biol Sci, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent513-522
dc.identifierhttp://dx.doi.org/10.1016/j.intimp.2012.08.009
dc.identifier.citationInternational Immunopharmacology. Amsterdam: Elsevier B.V., v. 14, n. 4, p. 513-522, 2012.
dc.identifier.doi10.1016/j.intimp.2012.08.009
dc.identifier.fileWOS000313152900022.pdf
dc.identifier.issn1567-5769
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35521
dc.identifier.wosWOS:000313152900022
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Immunopharmacology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectNattectinen
dc.subjectC-type lectinen
dc.subjectM1 activationen
dc.subjectMacrophagesen
dc.subjectTh1/Th2 cytokinesen
dc.titleRole of interplay between IL-4 and IFN-gamma in the in regulating M1 macrophage polarization induced by Nattectinen
dc.typeinfo:eu-repo/semantics/article
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