Sodium nitrite downregulates vascular NADPH oxidase and exerts antihypertensive effects in hypertension

dc.contributor.authorMontenegro, Marcelo Freitas
dc.contributor.authorAmaral, Jefferson Henrich
dc.contributor.authorPinheiro, Lucas Cézar
dc.contributor.authorSakamoto, Eliana Kiyomi
dc.contributor.authorFerreira, Graziele C.
dc.contributor.authorReis, Rosana Inacio dos [UNIFESP]
dc.contributor.authorMarcal, Diogo Machado de Oliveira
dc.contributor.authorPereira, Romaiana Picada
dc.contributor.authorTanus-Santos, Jose Eduardo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Sergipe (UFS)
dc.date.accessioned2016-01-24T14:16:54Z
dc.date.available2016-01-24T14:16:54Z
dc.date.issued2011-07-01
dc.description.abstractDietary nitrite and nitrate are important sources of nitric oxide (NO). However, the use of nitrite as an antihypertensive drug may be limited by increased oxidative stress associated with hypertension. We evaluated the antihypertensive effects of sodium nitrite given in drinking water for 4 weeks in two-kidney one-clip (21(1 C) hypertensive rats and the effects induced by nitrite on NO bioavailability and oxidative stress. We found that, even under the increased oxidative stress conditions present in 2K1C hypertension, nitrite reduced systolic blood pressure in a dose-dependent manner. Whereas treatment with nitrite did not significantly change plasma nitrite concentrations in 2K1C rats, it increased plasma nitrate levels significantly. Surprisingly, nitrite treatment exerted antioxidant effects in both hypertensive and sham-normotensive control rats. A series of in vitro experiments was carried out to show that the antioxidant effects induced by nitrite do not involve direct antioxidant effects or xanthine oxidase activity inhibition. Conversely, nitrite decreased vascular NADPH oxidase activity. Taken together, our results show for the first time that nitrite has antihypertensive effects in 2K1C hypertensive rats, which may be due to its antioxidant properties resulting from vascular NADPH oxidase activity inhibition. (C) 2011 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Fac Med Sci, Dept Pharmacol, BR-13081970 Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Med, Div Nephrol, BR-04023040 São Paulo, Brazil
dc.description.affiliationUniv Fed Santa Maria, Dept Chem, BR-97105900 Santa Maria, RS, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Med, Div Nephrol, BR-04023040 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPrograma Nacional de Cooperacao Academica
dc.format.extent144-152
dc.identifierhttp://dx.doi.org/10.1016/j.freeradbiomed.2011.04.005
dc.identifier.citationFree Radical Biology and Medicine. New York: Elsevier B.V., v. 51, n. 1, p. 144-152, 2011.
dc.identifier.doi10.1016/j.freeradbiomed.2011.04.005
dc.identifier.fileWOS000291628700014.pdf
dc.identifier.issn0891-5849
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33813
dc.identifier.wosWOS:000291628700014
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFree Radical Biology and Medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectNitriteen
dc.subjectAntioxidantsen
dc.subjectNADPH oxidaseen
dc.subjectNitric oxideen
dc.subjectXanthine oxidaseen
dc.subject2K1C hypertensionen
dc.subjectFree radicalsen
dc.titleSodium nitrite downregulates vascular NADPH oxidase and exerts antihypertensive effects in hypertensionen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
WOS000291628700014.pdf
Tamanho:
2.6 MB
Formato:
Adobe Portable Document Format
Descrição: