Neuroprotective effect of pyruvate and oxaloacetate during pilocarpine induced status epilepticus in rats

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dc.contributor.authorCarvalho, Andrezza Sossai Rodrigues [UNIFESP]
dc.contributor.authorTorres, Laila Brito [UNIFESP]
dc.contributor.authorPersike, Daniele Suzete [UNIFESP]
dc.contributor.authorFernandes, Maria Jose da Silva [UNIFESP]
dc.contributor.authorAmado, Débora [UNIFESP]
dc.contributor.authorNaffah-Mazzacoratti, Maria da Graca [UNIFESP]
dc.contributor.authorCavalheiro, Esper Abrão [UNIFESP]
dc.contributor.authorSilva, Alexandre Valotta da [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:06:05Z
dc.date.available2016-01-24T14:06:05Z
dc.date.issued2011-02-01
dc.description.abstractRecent research data have shown that systemic administration of pyruvate and oxaloacetate causes an increased brain-to-blood glutamate efflux. Since increased release of glutamate during epileptic seizures can lead to excitotoxicity and neuronal cell death, we tested the hypothesis that glutamate scavenging mediated by pyruvate and oxaloacetate systemic administration could have a neuroprotective effect in rats subjected to status epilepticus (SE). SE was induced by a single dose of pilocarpine (350 mg/kg i.p.). Thirty minutes after SE onset, a single dose of pyruvate (250 mg/kg i.p.), oxaloacetate (1.4 mg/kg i.p.), or both substances was administrated. Acute neuronal loss in hippocampal regions CA1 and hilus was quantitatively determined five hours after SE onset, using the optical fractionator method for stereological cell counting. Apoptotic cascade in the hippocampus was also investigated seven days after SE using caspase-1 and -3 activity assays. SE-induced neuronal loss in CA1 was completely prevented in rats treated with pyruvate plus oxaloacetate. the SE-induced caspase-1 activation was significantly reduced when rats were treated with oxaloacetate or pyruvate plus oxaloacetate. the treatment with pyruvate and oxaloacetate caused a neuroprotective effect in rats subjected to pilocarpine-induced SE. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNIFESP São Paulo, Dept Neurol & Neurosurg, São Paulo, Brazil
dc.description.affiliationUNIFESP Santos, Dept Biosci, Santos, SP, Brazil
dc.description.affiliationUnifespUNIFESP São Paulo, Dept Neurol & Neurosurg, São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP Santos, Dept Biosci, Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent385-390
dc.identifierhttp://dx.doi.org/10.1016/j.neuint.2010.12.014
dc.identifier.citationNeurochemistry International. Oxford: Pergamon-Elsevier B.V., v. 58, n. 3, p. 385-390, 2011.
dc.identifier.doi10.1016/j.neuint.2010.12.014
dc.identifier.fileWOS000295749500015.pdf
dc.identifier.issn0197-0186
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33383
dc.identifier.wosWOS:000295749500015
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNeurochemistry International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectBrainen
dc.subjectHippocampusen
dc.subjectStereologyen
dc.subjectEpilepsyen
dc.titleNeuroprotective effect of pyruvate and oxaloacetate during pilocarpine induced status epilepticus in ratsen
dc.typeinfo:eu-repo/semantics/article
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