Functional differences between two morphologically distinct cell subpopulations within a human colorectal carcinoma cell line

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dc.contributor.authorSolimene, A.c.c. [UNIFESP]
dc.contributor.authorCarneiro, Celia Regina Whitaker [UNIFESP]
dc.contributor.authorMelati, I. [UNIFESP]
dc.contributor.authorLopes, Jose Daniel [UNIFESP]
dc.contributor.institutionFundação Antônio Prudente
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:29:23Z
dc.date.available2015-06-14T13:29:23Z
dc.date.issued2001-05-01
dc.description.abstractThe LISP-I human colorectal adenocarcinoma cell line was isolated from a hepatic metastasis at the Ludwig Institute, São Paulo, SP, Brazil. The objective of the present study was to isolate morphologically different subpopulations within the LISP-I cell line, and characterize some of their behavioral aspects such as adhesion to and migration towards extracellular matrix components, expression of intercellular adhesion molecules and tumorigenicity in vitro. Once isolated, the subpopulations were submitted to adhesion and migration assays on laminin and fibronectin (crucial proteins to invasion and metastasis), as well as to anchorage-independent growth. Two morphologically different subpopulations were isolated: LISP-A10 and LISP-E11. LISP-A10 presents a differentiated epithelial pattern, and LISP-E11 is fibroblastoid, suggesting a poorly differentiated pattern. LISP-A10 expressed the two intercellular adhesion molecules tested, carcinoembryonic antigen (CEA) and desmoglein, while LISP-E11 expressed only low amounts of CEA. On the other hand, adhesion to laminin and fibronectin as well as migration towards these extracellular matrix proteins were higher in LISP-E11, as expected from its poorly differentiated phenotype. Both subpopulations showed anchorage-independent growth on a semi-solid substrate. These results raise the possibility that the heterogeneity found in the LISP-I cell line, which might have contributed to its ability to metastasize, was due to at least two different subpopulations herein identified.en
dc.description.affiliationFundação Antônio Prudente
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e Parasitologia
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Microbiologia, Imunologia e Parasitologia
dc.description.sourceSciELO
dc.format.extent653-661
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2001000500014
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 5, p. 653-661, 2001.
dc.identifier.doi10.1590/S0100-879X2001000500014
dc.identifier.fileS0100-879X2001000500014.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2001000500014
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/1160
dc.identifier.wosWOS:000168735900014
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcarcinoembryonic antigenen
dc.subjectCEAen
dc.subjectcolorectal carcinoma cell lineen
dc.subjectlamininen
dc.subjectfibronectinen
dc.subjectdesmogleinen
dc.subjectmetastasisen
dc.titleFunctional differences between two morphologically distinct cell subpopulations within a human colorectal carcinoma cell lineen
dc.typeinfo:eu-repo/semantics/article
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