mRNA expression of corticotropin-releasing factor and urocortin 1 after restraint and foot shock together with alprazolam administration

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dc.contributor.authorCéspedes, Isabel Cristina [UNIFESP]
dc.contributor.authorOliveira, Amanda R. de
dc.contributor.authorSilva, Joelcimar M. da
dc.contributor.authorSilva, Andre V. da
dc.contributor.authorSita, Luciane V.
dc.contributor.authorBittencourt, Jackson C.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:05:46Z
dc.date.available2016-01-24T14:05:46Z
dc.date.issued2010-12-01
dc.description.abstractCorticotropin-releasing factor (CRF) is expressed in the paraventricular nucleus of the hypothalamus (PVN), and act centrally to provoke stress-like autonomic and behavioral responses. Urocortins 1-3 are additional ligands to the CRF receptors 1 and 2. Ucn 1 neurons are primarily concentrated in the Edinger-Westphal (EW) nucleus and also have been associated with stress responses. It is also known that UCN 1 respond in different ways depending on the stressor presented. Benzodiazepines can act via the CRF peptidergic system and chronic administration of alprazolam does not interfere with CRF mRNA expression in the PVN, but significantly increase Ucn 1 mRNA expression in the EW. the aim of our study was to investigate the relationship between different stressor stimuli, foot shock (FS) and restraint (R), and the mRNA expression of CRF and Ucn 1 in the PVN and EW using alprazolam (A). We employed fos activation and in situ hybridization. Restraint group presented increased fos-ir and CRF mRNA expression in the PVN compared to FS group. the stress responses of R group were prevented by A. in the EW,fos-ir was higher in the FS group than in the R group, whereas Ucn 1 mRNA expression was higher in the R group than in the FS group. Alprazolam significantly increased fos-ir and Ucn 1 mRNA expression in both groups. Our results show that PVN and EW respond in different ways to the same stressors. Furthermore, EW of stressed animals replies in a complementary way comparing to PVN with the use of Alprazolam. (C) 2010 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biosci, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Lab Chem Neuroanat, Dept Anat, Inst Biomed Sci, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Psychol, Ctr Neurosci & Behav, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biosci, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 04/13849-5
dc.format.extent2200-2208
dc.identifierhttps://dx.doi.org/10.1016/j.peptides.2010.08.022
dc.identifier.citationPeptides. New York: Elsevier B.V., v. 31, n. 12, p. 2200-2208, 2010.
dc.identifier.doi10.1016/j.peptides.2010.08.022
dc.identifier.issn0196-9781
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/33134
dc.identifier.wosWOS:000284723400007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPeptides
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectStressen
dc.subjectCRF receptorsen
dc.subjectBenzodiazepinesen
dc.subjectAnxietyen
dc.subjectUrocortinsen
dc.subjectParaventricular nucleus of the hypothalamusen
dc.subjectEdinger-Westphal nucleusen
dc.titlemRNA expression of corticotropin-releasing factor and urocortin 1 after restraint and foot shock together with alprazolam administrationen
dc.typeinfo:eu-repo/semantics/article
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