HSP70 induced by hyperosmotic stress partially protects LLC-PK1 cells against nephrotoxic drugs

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dc.contributor.authorLeitao, A. A.
dc.contributor.authorLeite, C. A.
dc.contributor.authorSantos, B. C.
dc.contributor.authorBoim, M. A.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:56Z
dc.date.available2016-01-24T12:33:56Z
dc.date.issued2003-07-01
dc.description.abstractAccumulation of HSP70 is related to the cytoprotection. It was evaluated whether hyperosmotic stress induces HSP70 accumulation in LLC-PK1 cells, and protects cells against toxicity provoked by cisplatin (Cis) and cyclosporine A (CyA). Cells were maintained in isosmotic (Iso) or hyperosmotic (H) culture medium for 24 h and then exposed to Cis or CyA for an additional period of 12 or 24 h (groups H+Cis and H+CyA). the H medium did not induce cell death and increased both HSP70 mRNA and protein levels, suggesting a role in cell adaptation to H condition. H medium produced partial cytoprotection against Cis and CyA compared with control cells. Despite the cytoprotection, there was a reduction in HSP70 mRNA and protein levels in H+Cis group. in contrast, the H+CyA group presented high levels of HSP70 mRNA and protein. the induction of HSP70 by H medium was associated with tolerance of LLC-PK1 cells against Cis and CyA cytotoxicity but this protection was induced by different mechanisms and depended on the characteristics of the drug used. Copyright (C) 2003 S. Karger AG, Basel.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Div Nephrol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extentE94-E102
dc.identifierhttp://dx.doi.org/10.1159/000072027
dc.identifier.citationNephron Experimental Nephrology. Basel: Karger, v. 94, n. 3, p. E94-E102, 2003.
dc.identifier.doi10.1159/000072027
dc.identifier.issn1660-2129
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27319
dc.identifier.wosWOS:000186538000003
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofNephron Experimental Nephrology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjecthyperosmotic stressen
dc.subjectcisplatinen
dc.subjectcyclosporineen
dc.subjectLLC-PK1 cellsen
dc.titleHSP70 induced by hyperosmotic stress partially protects LLC-PK1 cells against nephrotoxic drugsen
dc.typeinfo:eu-repo/semantics/article
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