Effect of nitric oxide on excitatory amino acid-evoked discharge of neurons in NTS

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dc.contributor.authorDias, ACR
dc.contributor.authorColombari, E.
dc.contributor.authorMifflin, S. W.
dc.contributor.institutionUniv Texas
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:38Z
dc.date.available2016-01-24T12:33:38Z
dc.date.issued2003-01-01
dc.description.abstractN-methyl-D-aspartate (NMDA) and non-NMDA excitatory amino acid (EAA) receptor subtypes are involved in the integration of visceral afferent inputs within the nucleus of the solitary tract (NTS). Microinjection studies indicate interactions between nitric oxide (NO) and EAA receptors within the NTS. To examine these interactions at the single cell level, this study characterized the effects of the NO synthase inhibitor N-G-nitro-L-arginine methyl ester (L-NAME) and the NO donor 3-[2-hydroxy-2-nitroso-1-propylhydrazino]-1-propanamine (PAPA-NONOate) on the excitatory responses of vagus nerve (VN)-evoked NTS neurons to the activation of (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and NMDA receptors in rats. Iontophoresis of L-NAME did not alter spontaneous or VN-evoked discharges, but significantly decreased the number of action potentials (APs) evoked by iontophoretic application of AMPA. the effects of L-NAME on NMDA-evoked discharge were variable; for the population, L-NAME did not change the number of APs evoked by NMDA. PAPA-NONOate enhanced the spontaneous discharge and the number of APs elicited by AMPA but not NMDA. Iontophoresis of the inactive enantiomers N-G-nitro-D-arginine methyl ester and hydroxydiazenesulfonic acid 1-oxide disodium salt had no effect on AMPA-evoked discharge. Our data suggest that NO facilitates AMPA-mediated neuronal transmission within the NTS.en
dc.description.affiliationUniv Texas, Hlth Sci Ctr, Dept Pharmacol, San Antonio, TX 78229 USA
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Physiol, BR-04023060 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Physiol, BR-04023060 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extentH234-H240
dc.identifierhttp://dx.doi.org/10.1152/ajpheart.00037.2002
dc.identifier.citationAmerican Journal of Physiology-heart and Circulatory Physiology. Bethesda: Amer Physiological Soc, v. 284, n. 1, p. H234-H240, 2003.
dc.identifier.doi10.1152/ajpheart.00037.2002
dc.identifier.issn0363-6135
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27082
dc.identifier.wosWOS:000179857900028
dc.language.isoeng
dc.publisherAmer Physiological Soc
dc.relation.ispartofAmerican Journal of Physiology-heart and Circulatory Physiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectnucleus of the solitary tracten
dc.subjectN-methyl-D-aspartateen
dc.subject(RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic aciden
dc.subjectvagus nerve stimulationen
dc.titleEffect of nitric oxide on excitatory amino acid-evoked discharge of neurons in NTSen
dc.typeinfo:eu-repo/semantics/article
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