Avaliação da proliferação celular e apoptose em células da mucosa bucal de ratos expostos ao decanoato de nandrolona
Data
2012-12-03
Autores
Pozzi, Renan 
Orientadores
Ribeiro, Daniel Araki
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: Verificar a interferência do decanoato de nandrolona em proteínas
regulatórias relacionadas à proliferação celular, apoptose e COX-2 em células da
mucosa bucal de ratos Wistar.
Metodologia: Um total de 40 ratos foi distribuído em quatro grupos. Dois grupos
experimentais foram tratados com decanoato de nandrolona, na dose de 5mg/kg, via
subcutânea, três vezes por semana em dois períodos experimentais: 15 e 30 dias (G3
e G4, respectivamente). Os demais grupos (G1 e G2), receberam somente solução
salina a 0,9% via subcutânea, três vezes por semana. Após os períodos experimentais,
os animais foram submetidos à eutanásia e as línguas coletadas para análise
morfológica e de marcadores relacionados à proliferação, apoptose e inflamação (ki67, p53 e COX-2) por meio da técnica de imunoistoquímica.
Resultados: Na análise histopatológica, não foram observadas modificações
morfológicas no tecido lingual em todos os grupos avaliados. Entretanto, um aumento
significativo na imunoexpressão das proteínas ki-67, p53 e COX-2 foi detectado nos
grupos tratados com decanoato de nandrolona em 15 e 30 dias quando comparados
aos seus respectivos controles. Além disso, houve correlação tempo-resposta para a
proteína ki-67, a qual se evidenciou aumento significativo. Encontrou-se também
correlação positiva entre a imunoexpressão das proteínas p53 e COX-2 nos dois
momentos experimentais avaliados.
Conclusão: Nossos resultados sugerem que o decanoato de nandrolona foi capaz de
alterar a expressão de proteínas relacionadas ao ciclo celular, bem como induzir a
imunoexpressão de COX-2 em células da língua de ratos Wistar. Foi confirmada a
correlação positiva de tempo-resposta para a proteína ki-67 e entre a imunoexpressão
das proteínas COX-2 e p53.
Objectives: To evaluate the impact potential of nandrolone decanoate on cellular regulatory proteins, Ki-67 and p53, and COX-2 in oral mucosa cells of Wistar rats. Methods: A total of 40 rats were distributed into four groups. Two experimental groups were treated with nandrolone decanoate, at 5mg/kg dose, subcutaneously, three times a week during two periods: 15 and 30 days (G3 and G4, respectively). The remaining groups (G1 and G2) received only 0.9% saline subcutaneously three times a week. After treatment period, all animals underwent euthanasia and their tongues were collected to analyze morphology and immunomarkers related to proliferation, apoptosis and inflammation (Ki-67, p53 and COX-2) by immunohistochemistry. Results: In the histopathological analysis, no morphological changes were observed in tongue tissue for all groups. Significant increase in Ki-67, p53, COX-2 immunoexpression was detected in the groups treated with nandrolone decanoate during 15 and 30 days, when compared to their respective controls. There was timeresponse relationship for Ki-67 protein. Furthermore, positive correlation between immunoexpression of p53 and COX-2 protein was found. Conclusion: Our results suggest that nandrolone decanoate was able to impair the expression of cell cycle related proteins, as well as induce COX-2 immunoexpression in tongue cells of Wistar rats. There was confirmed positive correlation between timeresponse and ki-67, and between immunoexpression of p53 and COX-2 protein.
Objectives: To evaluate the impact potential of nandrolone decanoate on cellular regulatory proteins, Ki-67 and p53, and COX-2 in oral mucosa cells of Wistar rats. Methods: A total of 40 rats were distributed into four groups. Two experimental groups were treated with nandrolone decanoate, at 5mg/kg dose, subcutaneously, three times a week during two periods: 15 and 30 days (G3 and G4, respectively). The remaining groups (G1 and G2) received only 0.9% saline subcutaneously three times a week. After treatment period, all animals underwent euthanasia and their tongues were collected to analyze morphology and immunomarkers related to proliferation, apoptosis and inflammation (Ki-67, p53 and COX-2) by immunohistochemistry. Results: In the histopathological analysis, no morphological changes were observed in tongue tissue for all groups. Significant increase in Ki-67, p53, COX-2 immunoexpression was detected in the groups treated with nandrolone decanoate during 15 and 30 days, when compared to their respective controls. There was timeresponse relationship for Ki-67 protein. Furthermore, positive correlation between immunoexpression of p53 and COX-2 protein was found. Conclusion: Our results suggest that nandrolone decanoate was able to impair the expression of cell cycle related proteins, as well as induce COX-2 immunoexpression in tongue cells of Wistar rats. There was confirmed positive correlation between timeresponse and ki-67, and between immunoexpression of p53 and COX-2 protein.
Descrição
Citação
POZZI, Renan. Avaliação da proliferação celular e apoptose em células da mucosa bucal de ratos expostos ao decanoato de nandrolona. 2012. 92f. Dissertação (Mestrado) - Instituto de Saúde e Sociedade, Universidade Federal de São Paulo, Santos, 2012.