Glycosaminoglycans from aged human hippocampus have altered capacities to regulate trophic factors activities but not A beta 42 peptide toxicity

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dc.contributor.authorMinh Bao Huynh
dc.contributor.authorVillares, Joao [UNIFESP]
dc.contributor.authorDiaz, Julia Elisa Sepulveda
dc.contributor.authorChristiaans, Stephy
dc.contributor.authorCarpentier, Gilles
dc.contributor.authorOuidja, Mohand Ouidir
dc.contributor.authorSissoeff, Ludmilla
dc.contributor.authorRaisman-Vozari, Rita
dc.contributor.authorPapy-Garcia, Dulce
dc.contributor.institutionUniv Paris Est
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Paris 06
dc.date.accessioned2016-01-24T14:27:12Z
dc.date.available2016-01-24T14:27:12Z
dc.date.issued2012-05-01
dc.description.abstractGlycosaminoglycans (GAGs) are major extracellular matrix components known to tightly regulate cell behavior by interacting with tissue effectors as trophic factors and other heparin binding proteins. Alterations of GAGs structures might thus modify the nature and extent of these interactions and alter tissue integrity. Here, we studied levels and composition of GAGs isolated from adult and aged human hippocampus and investigated if their changes can influence the function of important trophic factors and the A beta 42 peptide toxicity. Biochemical analyses showed that heparan sulfates are increased in the aged hippocampus. Moreover, GAGs from aged hippocampus showed altered capacities to regulate trophic factor activities without changing their capacities to protect cells from A beta 42 toxicity, compared to adult hippocampus GAGs. Structural alterations in GAGs from elderly were suggested by differential transcripts levels of key biosynthetic enzymes. C5-epimerase and 2-OST expressions were decreased while NDST-2 and 3-OST-4 were increased; in contrast, heparanase expression was unchanged. Results suggest that alteration of GAGs in hippocampus of aged subjects could participate to tissue impairment during aging. (C) 2012 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniv Paris Est, Fac Sci & Technol, CNRS, Lab CRRET,EAC 7149, F-94010 Creteil, France
dc.description.affiliationUniversidade Federal de São Paulo, Aging & Neurodegenerat Dis Brain Bank Invest Lab, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniv Paris 06, INSERM, UMR S 975, UPMC,CRICM, F-75013 Paris, France
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Aging & Neurodegenerat Dis Brain Bank Invest Lab, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFrench Ministry of Superior Education and Research
dc.format.extent12
dc.identifierhttp://dx.doi.org/10.1016/j.neurobiolaging.2011.09.030
dc.identifier.citationNeurobiology of Aging. New York: Elsevier B.V., v. 33, n. 5, 12 p., 2012.
dc.identifier.doi10.1016/j.neurobiolaging.2011.09.030
dc.identifier.issn0197-4580
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34862
dc.identifier.wosWOS:000302486200025
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofNeurobiology of Aging
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectAgingen
dc.subjectHippocampusen
dc.subjectExtracellular matrixen
dc.subjectGlycosaminoglycansen
dc.subjectHeparan sulfateen
dc.subjectGrowth factorsen
dc.subjectA betaen
dc.titleGlycosaminoglycans from aged human hippocampus have altered capacities to regulate trophic factors activities but not A beta 42 peptide toxicityen
dc.typeinfo:eu-repo/semantics/article
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