Effect of D-alpha-tocopherol on tubular nephron acidification by rats with induced diabetes mellitus

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dc.contributor.authorGomes, G. Nascimento [UNIFESP]
dc.contributor.authorBarbosa, F.t. [UNIFESP]
dc.contributor.authorRadaeli, R.f. [UNIFESP]
dc.contributor.authorCavanal, Maria de Fátima [UNIFESP]
dc.contributor.authorAires, M. Mello
dc.contributor.authorGil, Frida Zaladek [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-06-14T13:31:39Z
dc.date.available2015-06-14T13:31:39Z
dc.date.issued2005-07-01
dc.description.abstractThe objective of the present study was to determine if treatment of diabetic rats with D-alpha-tocopherol could prevent the changes in glomerular and tubular function commonly observed in this disease. Sixty male Wistar rats divided into four groups were studied: control (C), control treated with D-alpha-tocopherol (C + T), diabetic (D), and diabetic treated with D-alpha-tocopherol (D + T). Treatment with D-alpha-tocopherol (40 mg/kg every other day, ip) was started three days after diabetes induction with streptozotocin (60 mg/kg, ip). Renal function studies and microperfusion measurements were performed 30 days after diabetes induction and the kidneys were removed for morphometric analyses. Data are reported as means ± SEM. Glomerular filtration rate increased in D rats but decreased in D + T rats (C: 6.43 ± 0.21; D: 7.74 ± 0.45; D + T: 3.86 ± 0.18 ml min-1 kg-1). Alterations of tubular acidification observed in bicarbonate absorption flux (JHCO3) and in acidification half-time (t/2) in group D were reversed in group D + T (JHCO3, C: 2.30 ± 0.10; D: 3.28 ± 0.22; D + T: 1.87 ± 0.08 nmol cm-2 s-1; t/2, C: 4.75 ± 0.20; D: 3.52 ± 0.15; D + T: 5.92 ± 0.19 s). Glomerular area was significantly increased in D, while D + T rats exhibited values similar to C, suggesting that the vitamin prevented the hypertrophic effect of hyperglycemia (C: 8334.21 ± 112.05; D: 10,217.55 ± 100.66; D + T: 8478.21 ± 119.81µm²). These results suggest that D-alpha-tocopherol is able to protect rats, at least in part, from the harmful effects of diabetes on renal function.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Fisiologia
dc.description.affiliationUniversidade de São Paulo Instituto de Ciências Biomédicas Departamento de Fisiologia e Biofísica
dc.description.affiliationUnifespUNIFESP, EPM, Depto. de Fisiologia
dc.description.sourceSciELO
dc.format.extent1043-1051
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2005000700007
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 7, p. 1043-1051, 2005.
dc.identifier.doi10.1590/S0100-879X2005000700007
dc.identifier.fileS0100-879X2005000700007.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2005000700007
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/2591
dc.identifier.wosWOS:000230829900007
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDiabetesen
dc.subjectD-alpha-tocopherolen
dc.subjectRenal functionen
dc.subjectNephropathyen
dc.titleEffect of D-alpha-tocopherol on tubular nephron acidification by rats with induced diabetes mellitusen
dc.typeinfo:eu-repo/semantics/article
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