Oropouche virus entry into HeLa cells involves clathrin and requires endosomal acidification

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dc.contributor.authorSantos, Rodrigo I. M.
dc.contributor.authorRodrigues, Alcir H.
dc.contributor.authorSilva, Maria Lucia
dc.contributor.authorMortara, Renato A. [UNIFESP]
dc.contributor.authorRossi, Marcos A.
dc.contributor.authorJamur, Maria Celia
dc.contributor.authorOliver, Constance
dc.contributor.authorArruda, Eurico
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:51:57Z
dc.date.available2016-01-24T13:51:57Z
dc.date.issued2008-12-01
dc.description.abstractOropouche virus (ORO), family Bunyaviridae, is the second most frequent cause of arboviral febrile illness in Brazil. Studies were conducted to understand ORO entry in HeLa cells. Chlorpromazine inhibited early steps of ORO replication cycle, consistent with entry/uncoating. the data indicate that ORO enters HeLa cells by clathrin-coated vesicles, by a mechanism susceptible to endosomal acidification inhibitors. Transmission electron microscopy and immunofluorescence indicated that ORO associates with clathrin-coated pits and can be found in association with late endosomes in a time shorter than 1 h. (C) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Sch Med Ribeirao Preto, Dept Cell & Mol Biol, BR-05508 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med Ribeirao Preto, Dept Pathol, BR-05508 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFAPFSP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPFSP: 2003/03682-3
dc.format.extent139-143
dc.identifierhttp://dx.doi.org/10.1016/j.virusres.2008.08.016
dc.identifier.citationVirus Research. Amsterdam: Elsevier B.V., v. 138, n. 1-2, p. 139-143, 2008.
dc.identifier.doi10.1016/j.virusres.2008.08.016
dc.identifier.issn0168-1702
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31084
dc.identifier.wosWOS:000261586900018
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVirus Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectOropouche virusen
dc.subjectClathrinen
dc.subjectEndocytosisen
dc.subjectBunyaviridaeen
dc.titleOropouche virus entry into HeLa cells involves clathrin and requires endosomal acidificationen
dc.typeinfo:eu-repo/semantics/article
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