Binding of laminin to Paracoccidioides brasiliensis induces a less severe pulmonary paracoccidioidomycosis caused by virulent and low-virulence isolates

Página do item simplificado
dc.contributor.authorAndre, D. C.
dc.contributor.authorLopes, J. D.
dc.contributor.authorFranco, M. F.
dc.contributor.authorVaz, CAC
dc.contributor.authorCalich, VLG
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:37:08Z
dc.date.available2016-01-24T12:37:08Z
dc.date.issued2004-05-01
dc.description.abstractThe pathogenic fungus Parracoccidioides brasiliensis is the causative agent of paraeoccidioidomycosis (PCM). This pulmonary mycosis, acquired by inhalation of airborne propagules, may disseminate to several internal organs and tissues, leading to severe disease. Adhesion to host cell components is the first step involved in dissemination of pathogens. Previous studies showed that laminin, the most abundant glycoprotein of the basement membrane, binds to P. brasiliensis yeast cells, enhancing their pathogenicity in the hamster testicle model. As PCM is primarily a pulmonary infection, we studied the influence of previous treatment of yeast cells with laminin on the course of the intratracheal infection of resistant and susceptible mice using high-virulence (Pb18) and low-virulence (Pb265) P. brasiliensis isolates. Laminin treatment did not alter fungal loads, delayed-type hypersensitivity reactions, levels of pulmonary cytokines and production of specific antibodies in any group of Pb18-infected mice. However, early in the infection, a less intense inflammatory reaction was detected in the lungs of the laminin-treated groups. in addition, laminin treatment of P6265 resulted in a less severe infection as revealed by the lower fungal loads recovered from lungs. Antibody and cytokine levels, however, did not change after laminin treatment. Altogether, our results demonstrate that laminin binding to yeast cells diminishes P. brasiliensis pathogenicity. the lower inflammatory response observed with the virulent isolate and the decreased pulmonary fungal burden with the low-virulence isolate indicate an inhibitory effect of laminin treatment on P. brasiliensis infectivity and interaction with pulmonary host cells or extracellular matrix proteins. (C) 2004 Elsevier SAS. All rights reserved.en
dc.description.affiliationUniv São Paulo, Inst Ciencias Biomed, Dept Immunol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Fac Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Fac Med, Dept Patol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fac Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fac Med, Dept Patol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent549-558
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2004.02.010
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 6, n. 6, p. 549-558, 2004.
dc.identifier.doi10.1016/j.micinf.2004.02.010
dc.identifier.issn1286-4579
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27733
dc.identifier.wosWOS:000221835000005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectParacoccidioides brasiliensisen
dc.subjectlamininen
dc.subjectpathogenicityen
dc.subjectimmunityen
dc.titleBinding of laminin to Paracoccidioides brasiliensis induces a less severe pulmonary paracoccidioidomycosis caused by virulent and low-virulence isolatesen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral