Interleukin-6-174 G/C promoter polymorphism and nasal polyposis

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dc.contributor.authorKosugi, Eduardo Macoto [UNIFESP]
dc.contributor.authorCamargo-Kosugi, Cintia Meirelles de [UNIFESP]
dc.contributor.authorMaurice Weckx, Luc Louis [UNIFESP]
dc.contributor.authorSilva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
dc.contributor.authorGregorio, Luiz Carlos [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-18T11:54:41Z
dc.date.available2018-06-18T11:54:41Z
dc.date.issued2009-12-01
dc.description.abstractIntroduction: Nasal polyposis is a chronic disease with unknown etiopathogenesis, although inflammatory mechanisms seem to play a role. One of several inflammatory mediators linked to nasal polyposis is Interleukin-6, which has a single. nucleotide polymorphism -174 G/C that seems to promote an inflammatory reaction.Objective: To compare the prevalence of the -174 G/C single nucleotide polymorphism between a group of patients with nasal polyposis and a control group.Method. Cross-sectional study with two groups (thirty two patients with nasal polyposis and fifty five controls) to investigate the -174 G/C polymorphism in blood samples. Asthma, aspirin intolerance and atopy were main exclusion criteria. IL-6 genotyping was performed using the PCR method with forward primer 5'-ATGCCAAGTGCTGAGTCACTA-3' and reverse primer 5'-GGAAAATCCCACATTTGATA-3', amplifying a 226-bp DNA fragment that contained the 174 position. The amplified fragment can be cleaved by restriction enzyme NlaIII when the -174 position presented the C allele in two fragments of 117 and 109-bp, visualized by electrophoresis, classifying participants in GG, GC and CC.Results: In the nasal polyposis group, 65.62% of the patients had the GG genotype, while in the control group only 41.82% had the GG genotype, a statistically significant difference, with an odds ratio of 2.65.Conclusion: The -174 GG genotype was found more frequency in nasal polyposis patients than in controls, when asthma, aspirin intolerance and atopy were excluded.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, EPM, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Ginecol, EPM, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, EPM, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Ginecol, EPM, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent400-404
dc.identifierhttp://www.rhinologyjournal.com/Rhinology_issues/807.pdf
dc.identifier.citationRhinology. Utrecht: Int Rhinologic Soc, v. 47, n. 4, p. 400-404, 2009.
dc.identifier.issn0300-0729
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/45527
dc.identifier.wosWOS:000273234200015
dc.language.isoeng
dc.publisherInt Rhinologic Soc
dc.relation.ispartofRhinology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectnasal polypsen
dc.subjectinterleukin-6en
dc.subjectgenetic polymorphismen
dc.subjectrestriction fragment length polymorphismen
dc.subjectsingle nucleotide polymorphismen
dc.titleInterleukin-6-174 G/C promoter polymorphism and nasal polyposisen
dc.typeinfo:eu-repo/semantics/article
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