Bothrops jararaca venom proteome rearrangement upon neonate to adult transition

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dc.contributor.authorZelanis, Andre
dc.contributor.authorTashima, Alexandre Keiji [UNIFESP]
dc.contributor.authorPinto, Antônio Frederico Michel
dc.contributor.authorPaes Leme, Adriana Franco
dc.contributor.authorStuginski, Daniel Rodrigues
dc.contributor.authorFurtado, Maria de Fatima Domingues
dc.contributor.authorSherman, Nicholas E.
dc.contributor.authorHo, Paulo Lee
dc.contributor.authorFox, Jay W.
dc.contributor.authorSerrano, Solange Maria de Toledo
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionCtr Biotecnol UFRGS
dc.contributor.institutionLNBio
dc.contributor.institutionUniv Virginia
dc.date.accessioned2016-01-24T14:17:22Z
dc.date.available2016-01-24T14:17:22Z
dc.date.issued2011-11-01
dc.description.abstractThe pharmacological activities displayed by Bothrops jararaca venom undergo a significant ontogenetic shift. Similarly, the diet of this species changes from ectothermic prey in early life to endothermic prey in adulthood. in this study we used large and representative newborn and adult venom samples consisting of pools from 694 and 110 specimens, respectively, and demonstrate a significant ontogenetic shift in the venom proteome complexity of B. jararaca. 2-DE coupled to MS protein identification showed a clear rearrangement of the toxin arsenal both in terms of the total proteome, as of the glycoproteome. N-glycosylation seems to play a key role in venom protein variability between newborn and adult specimens. Upon the snake development, the subproteome of metalloproteinases undergoes a shift from a P-III-rich to a P-I-rich profile while the serine proteinase profile does not vary significantly. We also used isobaric tag labeling (iTRAQ) of venom tryptic peptides for the first time to examine the quantitative changes in the venom toxins of B. jararaca upon neonate to adult transition. the iTRAQ analysis showed changes in various toxin classes, especially the proteinases. Our study expands the in-depth understanding of venom complexity variation particularly with regard to toxin families that have been associated with envenomation pathogenesis.en
dc.description.affiliationInst Butantan, Lab Especial Toxinol Aplicada CAT Cepid, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Bioquim, Inst Quim, BR-05508 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, São Paulo, Brazil
dc.description.affiliationCtr Biotecnol UFRGS, Lab Bioquim Farmacol, Porto Alegre, RS, Brazil
dc.description.affiliationLNBio, Lab Nacl Biociencias, Campinas, Brazil
dc.description.affiliationInst Butantan, Lab Herpetol, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv Virginia, Dept Microbiol, Charlottesville, VA 22908 USA
dc.description.affiliationInst Butantan, Ctr Biotecnol, BR-05503900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipRede de Proteoma de São Paulo
dc.description.sponsorshipIDFAPESP: 07/54626-7
dc.description.sponsorshipIDFAPESP: 98/14307-9
dc.description.sponsorshipIDRede de Proteoma de São Paulo: FAPESP 2004/14846-0/FINEP 01.07.0290.00
dc.format.extent4218-4228
dc.identifierhttp://dx.doi.org/10.1002/pmic.201100287
dc.identifier.citationProteomics. Hoboken: Wiley-Blackwell, v. 11, n. 21, p. 4218-4228, 2011.
dc.identifier.doi10.1002/pmic.201100287
dc.identifier.issn1615-9853
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34178
dc.identifier.wosWOS:000297223200009
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofProteomics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectAnimal proteomicsen
dc.subjectGlycosylationen
dc.subjectiTRAQen
dc.subjectOntogenetic venom variationen
dc.subjectSnake venomen
dc.titleBothrops jararaca venom proteome rearrangement upon neonate to adult transitionen
dc.typeinfo:eu-repo/semantics/article
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