Flavones from Erythrina falcata are modulators of fear memory

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dc.contributor.authorOliveira, Daniela Rodrigues de [UNIFESP]
dc.contributor.authorZamberlam, Claudia Raquel [UNIFESP]
dc.contributor.authorGaiardo, Renan Barreta [UNIFESP]
dc.contributor.authorRego, Gizelda Maia
dc.contributor.authorCerutti, Janete Maria [UNIFESP]
dc.contributor.authorCavalheiro, Alberto J.
dc.contributor.authorCerutti, Suzete Maria [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:37:42Z
dc.date.available2016-01-24T14:37:42Z
dc.date.issued2014-08-05
dc.description.abstractBackground: Flavonoids, which have been identified in a variety of plants, have been demonstrated to elicit beneficial effects on memory. Some studies have reported that flavonoids derived from Erythrina plants can provide such beneficial effects on memory. the aim of this study was to identify the flavonoids present in the stem bark crude extract of Erythrina falcata (CE) and to perform a bioactivity-guided study on conditioned fear memory.Methods: the secondary metabolites of CE were identified by high performance liquid chromatography combined with a diode array detector, electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn) and nuclear magnetic resonance (NMR). the buthanolic fraction (BuF) was obtained by partitioning. Subfractions from BuF (BuF1 - BuF6) and fraction flavonoidic (FfA and FfB) were obtained by flash chromatography. the BuF3 and BuF4 fractions were used for the isolation of flavonoids, which was performed using HPLC-PAD. the isolated substances were quantified by HPLC-DAD and their structures were confirmed by nuclear magnetic resonance (NMR). the activities of CE and the subfractions were monitored using a one-trial, step-down inhibitory avoidance (IA) task to identify the effects of these substances on the acquisition and extinction of conditioned fear in rats.Results: Six subclasses of flavonoids were identified for the first time in CE. According to our behavioral data, CE, BuF, BuF3 and BuF4, the flavonoidic fractions, vitexin, isovitexin and 6-C-glycoside-diosmetin improved the acquisition of fear memory. Rats treated with BuF, BuF3 and BuF4 were particularly resistant to extinction. Nevertheless, rats treated with FfA and FfB, vitexin, isovitexin and 6-C-glycoside-diosmetin exhibited gradual reduction in conditioned fear response during the extinction retest session, which was measured at 48 to 480 h after conditioning.Conclusions: Our results demonstrate that vitexin, isovitexin and diosmetin-6-C-glucoside and flavonoidic fractions resulted in a significant retention of fear memory but did not prevent the extinction of fear memory. These results further substantiate that the treatment with pure flavonoids or flavanoid-rich fractions might represent potential therapeutic approaches for the treatment of neurocognitive disorders, improvement of memory acquisition and spontaneous recovery of fear.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol Sci, Behav Pharmacol & Ethnopharmacol Lab, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol & Genet, Genet Bases Thyroid Tumor Lab, Div Genet, São Paulo, Brazil
dc.description.affiliationBrazilian Agr Res Corp EMBRAPA, Dept Forestry Colombo, Londrina, Parana, Brazil
dc.description.affiliationUNESP, State Univ São Paulo, Inst Chem, Nuclei Bioassay Biosynth & Ecophysiol Nat Prod Nu, Araraquara, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol Sci, Behav Pharmacol & Ethnopharmacol Lab, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol & Genet, Genet Bases Thyroid Tumor Lab, Div Genet, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBrazilian Agricultural Research Corporation (EMBRAPA)
dc.description.sponsorshipIDFAPESP: 2009/15229-8
dc.format.extent17
dc.identifierhttp://dx.doi.org/10.1186/1472-6882-14-288
dc.identifier.citationBmc Complementary and Alternative Medicine. London: Biomed Central Ltd, v. 14, 17 p., 2014.
dc.identifier.doi10.1186/1472-6882-14-288
dc.identifier.fileWOS000341109000003.pdf
dc.identifier.issn1472-6882
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38080
dc.identifier.wosWOS:000341109000003
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBmc Complementary and Alternative Medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFlavoneen
dc.subjectAcquisitionen
dc.subjectExtinctionen
dc.subjectFear conditioningen
dc.subjectErythrina falcata (Fabaceae)en
dc.subjectHPLC-ESI/MSnen
dc.titleFlavones from Erythrina falcata are modulators of fear memoryen
dc.typeinfo:eu-repo/semantics/article
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