Ovarian-Steroid Modulation of Locus Coeruleus Activity in Female Rats: Involvement in Luteinising Hormone Regulation

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dc.contributor.authorSzawka, R. E.
dc.contributor.authorRodovalho, G. V.
dc.contributor.authorMonteiro, P. M.
dc.contributor.authorCarrer, H. F.
dc.contributor.authorAnselmo-Franci, J. A. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionConsejo Nacl Invest Cient & Tecn
dc.date.accessioned2016-01-24T13:58:30Z
dc.date.available2016-01-24T13:58:30Z
dc.date.issued2009-07-01
dc.description.abstractThe noradrenergic nucleus locus coeruleus (LC) has been reported to regulate luteinising hormone (LH) secretion in female rats. Both oestrogen and progestin receptors have been demonstrated in LC neurones, suggesting that these cells are possibly responsive to variations in circulating levels of ovarian steroids. We therefore evaluated changes in the activity of LC neurones during the oestrous cycle and after ovarian-steroid treatment in ovariectomised (OVX) rats, as determined by immunoreactivity to Fos-related antigens (FRA), which comprises all of the known members of the Fos family. Effects of ovarian steroids on the firing rate of LC neurones were also determined in a slice preparation. the number of FRA/tyrosine hydroxylase (TH)-immunoreactive (ir) neurones in the LC increased from 14.00-16.00 h on pro-oestrus, coinciding with the onset of the LH surge and rise in plasma progesterone. FRA immunoreactivity was unaltered during dioestrus. Oestradiol-treated OVX rats (OVX+E) displayed marked reduction in FRA/TH-ir neurones in LC compared to oil-treated OVX rats. Accordingly, oestradiol superfusion significantly reduced the spontaneous firing rate of LC neurones in slices from OVX rats. Compared to OVX+E, oestradiol-treated rats injected with progesterone at 08.00 h (OVX+EP) exhibited higher number of FRA/TH-ir neurones in the LC at 10.00 h and 16.00 h, and great amplification of the LH surge. Bath application of progesterone significantly increased the spontaneous firing rate of OVX+E LC neurones. Our data suggest that ovarian steroids may physiologically modulate the activity of LC neurones in females, with possible implications for LH secretion. Moreover, oestradiol and progesterone appear to exert opposite and complementary effects (i.e. whereas oestradiol inhibits, progesterone, after oestradiol priming, stimulates LC activity).en
dc.description.affiliationUniversidade Federal de São Paulo, Fac Odontol Ribeirao Preto, Dept Morfol Estomatol & Fisiol, Neuroendocrinol Lab, BR-14040904 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Fisiol, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationConsejo Nacl Invest Cient & Tecn, INIMEC, Inst Invest Med Mercedes & Martin Ferreyra, Cordoba, Argentina
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fac Odontol Ribeirao Preto, Dept Morfol Estomatol & Fisiol, Neuroendocrinol Lab, BR-14040904 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent629-639
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2826.2009.01880.x
dc.identifier.citationJournal of Neuroendocrinology. Malden: Wiley-Blackwell Publishing, Inc, v. 21, n. 7, p. 629-639, 2009.
dc.identifier.doi10.1111/j.1365-2826.2009.01880.x
dc.identifier.issn0953-8194
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31664
dc.identifier.wosWOS:000266923800005
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Neuroendocrinology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectnoradrenalineen
dc.subjectoestrous cycleen
dc.subjectoestrogenen
dc.subjectprogesteroneen
dc.subjectFosen
dc.titleOvarian-Steroid Modulation of Locus Coeruleus Activity in Female Rats: Involvement in Luteinising Hormone Regulationen
dc.typeinfo:eu-repo/semantics/article
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