A non-anticoagulant heterofucan has antithrombotic activity in vivo

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dc.contributor.authorBarroso, Edjane Maria de Azevedo
dc.contributor.authorCosta, Leandro Silva
dc.contributor.authorMedeiros, Valquiria Pereira de [UNIFESP]
dc.contributor.authorCordeiro, Sara Lima
dc.contributor.authorCosta, Mariana Santana Santos Pereira
dc.contributor.authorFranco, Celia Regina Cavichiolo
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorLeite, Edda Lisboa
dc.contributor.authorRocha, Hugo Alexandre de Oliveira
dc.contributor.institutionUniv Fed Rio Grande do Norte
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Fed Parana
dc.date.accessioned2016-01-24T13:51:27Z
dc.date.available2016-01-24T13:51:27Z
dc.date.issued2008-06-01
dc.description.abstractFucan is a term used to denominate a family of sulfated L-fucose-rich polysaccharides. the brown alga Spatoglossum schroederi(Dictyotaceae) has three heterofucans namely fucan A, B and C. the 21 kDa fucan A is composed of a core of a beta (1-3) glucuronic acid-containing oligosaccharide of 4.5 kDa with branches at C4 of the fucose chains a (1-3) linked. the fucose is mostly substituted at C4 with a sulfate group and at C2 with chains of beta (1-4) xylose. This fucan has neither anticoagulant (from from 0.1 to 100 mu g) nor hemorrhagic activities (from 50 to 800 mu g/mL). the antithrombotic test in vivo showed that fucan A has no activity in any of the concentrations (from 0.2 to 20 mu g/g/day) tested 1 h after polysaccharide administration. However, when fucan A was injected endovenously 24 h before the ligature of the venae cavae, we observed a dose-dependent effect, reaching saturation at around 20 mu g/g of rat weight. in addition, this effect is also time-dependent, reaching saturation around 16 h after fucan administration. in addition, regardless of the administration route, fucan A displayed antithrombotic activity. the exception was the oral pathway. of particular importance was the finding that fucan A stimulates the synthesis of an antithrombotic heparan sulfate from endothelial cells like heparin. the hypothesis has been raised that the in vivo antithrombotic activity of fucan A is related to the increased production of this heparan. Taken together with the fact that the compound is practically devoid of anticoagulant and hemorrhagic activity, the data suggest that it may be an ideal antithrombotic agent in vivo.en
dc.description.affiliationUniv Fed Rio Grande do Norte, Dept Bioquim, Lab Biotecnol Polimeros Nat BIOPOL, Ctr Biociencias,Programa Posgrad Ciencias Saude, Natal, RN, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, Brazil
dc.description.affiliationUniv Fed Parana, Dept Biol Celular, BR-80060000 Curitiba, Parana, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent712-718
dc.identifierhttps://dx.doi.org/10.1055/s-2008-1074522
dc.identifier.citationPlanta Medica. Stuttgart: Georg Thieme Verlag Kg, v. 74, n. 7, p. 712-718, 2008.
dc.identifier.doi10.1055/s-2008-1074522
dc.identifier.issn0032-0943
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/30713
dc.identifier.wosWOS:000257284700002
dc.language.isoeng
dc.publisherGeorg Thieme Verlag Kg
dc.relation.ispartofPlanta Medica
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBrown algaeen
dc.subjectFucoidanen
dc.subjectAnticoagulant drugsen
dc.subjectAntithrombotic agentsen
dc.subjectDictyotaceaeen
dc.subjectSpatoglossum schroederien
dc.titleA non-anticoagulant heterofucan has antithrombotic activity in vivoen
dc.typeinfo:eu-repo/semantics/article
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