Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds

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dc.citation.volume4
dc.contributor.authorVizetto-Duarte, Catarina
dc.contributor.authorCustodio, Luisa
dc.contributor.authorAcosta, Gerardo
dc.contributor.authorLago, Joao H. G.M[UNIFESP]
dc.contributor.authorMorais, Thiago R. [UNIFESP]
dc.contributor.authorde Sousa, Carolina Bruno
dc.contributor.authorGangadhar, Katkam N.
dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorPereira, Hugo
dc.contributor.authorLima, Raquel T.
dc.contributor.authorHelena Vasconcelos, M.
dc.contributor.authorBarreiro, Luisa
dc.contributor.authorRauter, Amelia P.
dc.contributor.authorAlbericioi, Fernando
dc.contributor.authorVarela, Joao
dc.coverageLondon
dc.date.accessioned2020-08-21T17:00:23Z
dc.date.available2020-08-21T17:00:23Z
dc.date.issued2016
dc.description.abstractMarine organisms are a prolific source of drug leads in a variety of therapeutic areas. In the last few years, biomedical, pharmaceutical and nutraceutical industries have shown growing interest in novel compounds from marine organisms, including macroalgae. Cystoseira is a genus of Phaeophyceae (Fucales) macroalgae known to contain bioactive compounds. Organic extracts (hexane, diethyl ether, ethyl acetate and methanol extracts) from three Cystoseira species (C. humilis, C. tamariscifolia and C. usneoides) were evaluated for their total phenolic content, radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, and antiproliferative activity against a human hepatocarcinoma cell line (HepG2 cells). C. tamariscifolia had the highest TPC and RSA. The hexane extract of C. tamariscifolia (CTH) had the highest cytotoxic activity (IC50 = 2.31 mu g/mL), and was further tested in four human tumor (cervical adenocarcinoma HeLa; gastric adenocarcinoma AGS; colorectal adenocarcinoma HCT-15; neuroblastoma SH-SY5Y), and two non-tumor (murine bone marrow stroma S17 and human umbilical vein endothelial HUVEC) cell lines in order to determine its selectivity. CTH strongly reduced viability of all tumor cell lines, especially of HepG2 cells. Cytotoxicity was particularly selective for the latter cells with a selectivity index = 12.6 as compared to non-tumor cells. Incubation with CTH led to a 2-fold decrease of HepG2 cell proliferation as shown by the bromodeoxyuridine (BrdU) incorporation assay. CTH-treated HepG2 cells presented also pro-apoptotic features, such as increased Annexin Wpropidium iodide (PI) binding and dose-dependent morphological alterations in DAPI-stained cells. Moreover, it had a noticeable disaggregating effect on 3D multicellular tumor spheroids. Deme boxy cystoketal chromane, a derivative of the meroditerpenoid cystoketal, was identified as the active compound in CTH and was shown to display selective in vitro cYtotoxicitY towards HepG2 cells.en
dc.description.affiliationUniv Algarve, Fac Sci & Technol, Ctr Marine Sci, Campus Gambelas, Faro, Portugal
dc.description.affiliationInst Res Biomed Barcelona, Chem & Mol Pharmacol, Barcelona Sci Pk, Barcelona, Spain
dc.description.affiliationCIBER BNN, Networking Ctr Bioengn Biomat & Nanomed, Barcelona Sci Pk, Barcelona, Spain
dc.description.affiliationUniv Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Sao Paulo, Brazil
dc.description.affiliationUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-1200 Lisbon, Portugal
dc.description.affiliationUniv Porto, Inst Invest & Inovacao Saude, Rua Alfredo Allen, Oporto, Portugal
dc.description.affiliationUniv Porto, Canc Drug Resistance Grp, IPATIMUP Inst Mol Pathol & Immunol, Rua Julio Amaral Carvalho, Oporto, Portugal
dc.description.affiliationUniv Porto, Dept Pathol & Oncol, Fac Med, Alameda Prof Hernani Monteiro, Oporto, Portugal
dc.description.affiliationUniv Porto, Fac Pharm, Dept Biol Sci, Rua Jorge Viterbo Ferreira, Oporto, Portugal
dc.description.affiliationUniv Lisbon, Fac Sci, Dept Chem & Biochem, Ctr Chem & Biochem, P-1699 Lisbon, Portugal
dc.description.affiliationUniv Barcelona, Dept Organ Chem, Barcelona, Spain
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSEABIOMED
dc.description.sponsorshipXtremeBio
dc.description.sponsorshipFoundation for Science and Technology (FCT)
dc.description.sponsorshipPortuguese National Budget
dc.description.sponsorshipFCT
dc.description.sponsorshipCAPES
dc.description.sponsorshipCNPq
dc.description.sponsorshipIDSEABIOMED: PTDC/MAR/103957/2008
dc.description.sponsorshipIDXtremeBio: PTDC/MAR-EST/4346/2012
dc.description.sponsorshipIDFCT: CCMAR/Multi/04326/2013
dc.description.sponsorshipIDFCT :IF/00049/2012
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.7717/peerj.1704
dc.identifier.citationPeerj. London, v. 4, p. -, 2016.
dc.identifier.doi10.7717/peerj.1704
dc.identifier.fileWOS000370949200004.pdf
dc.identifier.issn2167-8359
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57971
dc.identifier.wosWOS:000370949200004
dc.language.isoeng
dc.publisherPeerj Inc
dc.relation.ispartofPeerj
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMarine natural productsen
dc.subjectBrown algaeen
dc.subjectAntioxidanten
dc.subjectAnti-hepatocarcinomaen
dc.subjectCystoseiraen
dc.titleCan macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compoundsen
dc.typeinfo:eu-repo/semantics/article
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