Porphyrin Distribution After Topical Aminolevulinic Acid in a Novel Porcine Model of Sebaceous Skin

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dc.contributor.authorSakamoto, Fernanda Hidemi [UNIFESP]
dc.contributor.authorTannous, Zeina
dc.contributor.authorDoukas, Apostolos G.
dc.contributor.authorFarinelli, William A.
dc.contributor.authorSmith, Nicholas A.
dc.contributor.authorZurakowski, David
dc.contributor.authorAnderson, R. Rox
dc.contributor.institutionHarvard Univ
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:52:12Z
dc.date.available2016-01-24T13:52:12Z
dc.date.issued2009-02-01
dc.description.abstractBackground and Objective: Aminolevulinic acid photodynamic therapy (ALA-PDT) depends on drug metabolism into porphyrins. Clinically, ALA-PDT has been used with a wide range of protocols for treating both epidermal and dermal targets, despite limited understanding of porphyrin biodistribution over time. We studied porphyrin accumulation after topical application of ALA in vivo, and also describe the porcine ear as a new animal model to study adnexal glands.Study Design/Materials and Methods: the microanatomy of anterior ear skin of swine was measured. Topical 20% ALA in water/ethanol was applied under occlusion. Biopsies taken after 5, 10, 15, and then every 15 minutes for a total of 3 hours were examined by fluorescence microscopy of frozen sections to assess accumulation and distribution of porphyrins.Results: Porphyrin fluorescence of digital photomicrograph images was not visually apparent until 3045 minutes after application, although quantitative pixel analysis showed a statistically significant increase in epidermal fluorescence only 15 minutes after ALA application. From 30 to 120 minutes, epidermis, hair follicles (HF), and sebaceous glands (SG) became progressively more fluorescent. Eccrine gland fluorescence began to be detected after 30 minutes; SG showed fluorescence starting at 45-75 minutes. Fluorescence in all sites reached maximum intensity from 75 to 180 minutes of incubation. There was a trend for HF and SG to express stronger fluorescence compared with epidermis and eccrine glands.Conclusion: Anterior pig ear skin is microanatomically similar to human sebaceous skin. the time-dependent accumulation of porphyrins in pilosebaceous units and eccrine glands in this model suggests other routes of uptake of topical ALA in addition to the trans-epidermal route. Apparently, time interval between ALA application and light exposure could be optimized for different uses of ALA-PDT. Lasers Surg. Med. 41:154-160, 2009. (C) 2009 Wiley-Liss, Inc.en
dc.description.affiliationHarvard Univ, Sch Med, Childrens Hosp Boston, Dept Surg, Boston, MA 02115 USA
dc.description.affiliationHarvard Univ, Massachusetts Gen Hosp, Sch Med, Wellman Ctr Photomed, Boston, MA 02114 USA
dc.description.affiliationHarvard Univ, Cambridge, MA 02138 USA
dc.description.affiliationHarvard Univ, Sch Med, Boston VA Hosp, Jamaica Pl, MA 02130 USA
dc.description.affiliationUniversidade Federal de São Paulo, Dept Dermatol, Escola Paulista Med UNIFESP EPM, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Dermatol, Escola Paulista Med UNIFESP EPM, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipWellman Center for Photomedicine in Boston, Massachusetts
dc.format.extent154-160
dc.identifierhttp://dx.doi.org/10.1002/lsm.20734
dc.identifier.citationLasers in Surgery and Medicine. Hoboken: Wiley-liss, v. 41, n. 2, p. 154-160, 2009.
dc.identifier.doi10.1002/lsm.20734
dc.identifier.issn0196-8092
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31257
dc.identifier.wosWOS:000263897200014
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofLasers in Surgery and Medicine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectacneen
dc.subjectanimal modelen
dc.subjectapocrine glandsen
dc.subjecteccrine glandsen
dc.subjectsebaceous glandsen
dc.subjectsweat glandsen
dc.subjecttime perceptionen
dc.subjectphysiologyen
dc.subjectphotodynamic therapyen
dc.titlePorphyrin Distribution After Topical Aminolevulinic Acid in a Novel Porcine Model of Sebaceous Skinen
dc.typeinfo:eu-repo/semantics/article
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