Immunoblotting of soluble antigens in Paracoccidioides brasiliensis culture

Carlos, Nilson de Jesus; Pinto, Debora Alves; Ono, Mario Augusto; Venancio, Emerson Jose; Camargo, Zoilo Pires de [UNIFESP]; Sano, Ayako; Itano, Eiko Nakagawa

Immunoblotting of soluble antigens in Paracoccidioides brasiliensis culture

Data

2014-03-01

Autores

Carlos, Nilson de Jesus

Pinto, Debora Alves

Ono, Mario Augusto

Venancio, Emerson Jose

Camargo, Zoilo Pires de

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Tipo

Artigo

Resumo

This study investigated the major soluble antigens produced by Paracoccidioides brasiliensis (Pb339) cultured in solid Sabouraud (pH 5.6 and 8.5), Sabouraud plus brain heart infusion and liquid tomato juice-enriched complex medium media at intervals of 3 days over 30 days by immunoblotting and concluded that, to optimize the source of each antigen, both time and growth conditions should be considered.

Citação

Microbiology and Immunology. Hoboken: Wiley-Blackwell, v. 58, n. 3, p. 212-214, 2014.