Beneficial effect of annexin A1 in a model of experimental allergic conjunctivitis

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dc.contributor.authorGimenes, Alexandre D. [UNIFESP]
dc.contributor.authorAndrade, Teresa Raquel M. [UNIFESP]
dc.contributor.authorMello, Claudia B.
dc.contributor.authorRamos, Lisandra [UNIFESP]
dc.contributor.authorGil, Cristiane D. [UNIFESP]
dc.contributor.authorOliani, Sonia M. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Estadual Paulista
dc.date.accessioned2016-01-24T14:40:27Z
dc.date.available2016-01-24T14:40:27Z
dc.date.issued2015-05-01
dc.description.abstractAnnexin A1 (ANXA1), a 37 kDa glucocorticoid-regulated protein, is a potent anti-inflammatory mediator effective in terminating acute inflammatory response, and its role in allergic settings has been poorly studied. the aim of this investigation was to evaluate the mechanism of action of ANXA1 in intraocular inflammation using a classical model of ovalbumin (OVA)-induced allergic conjunctivitis (AC). OVA-immunised Balb/c mice, wild-type (WT) and ANXA1-deficient (AnxA1(-/-)), were challenged with eye drops containing OVA on days 14-16 with a subset of WT animals pretreated intraperitoneally with the peptide AC(2-26) (N-terminal region of ANXA1) or dexamethasone (DEX). After 24 h of the last ocular challenge, WT mice treated with Ac2-26 and DEX had significantly reduced clinical signs of conjunctivitis (chemosis, conjunctival hyperaemia, lid oedema and tearing), plasma IgE levels, leukocyte (eosinophil and neutrophil) influx and mast cell degranulation in the conjunctiva compared to WT controls. These anti-inflammatory effects of DEX were associated with high endogenous levels of ANXA1 in the ocular tissues as detected by immunohistochemistry. Additionally, AC(2-26) administration was effective to reduce IL-2, IL-4, IL-10, IL-13, eotaxin and RANTES in the eye and lymph nodes compared to untreated WT animals. the lack of ANXA1 produced an exacerbated allergic response as detected by the density of the inflammatory cell influx to the conjunctiva and the cytokine/chemokine release. These different effects observed for Ac2-26 were correlated with diminished level of activated ERK at 24 h in the ocular tissues compared to untreated OVA group. Our findings demonstrate the protective effect of ANXA1 during the inflammatory allergic response suggesting this protein as a potential target for new ocular inflammation therapies. (C) 2015 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Histol Lab, Dept Morfol & Genet, BR-04023900 São Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Biol, Lab Imunomorfol, BR-15054000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Histol Lab, Dept Morfol & Genet, BR-04023900 São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2012/50641-0
dc.description.sponsorshipIDFAPESP: 2012/10637-3
dc.description.sponsorshipIDCNPq: CNPq - 302768/2010-6
dc.format.extent24-32
dc.identifierhttp://dx.doi.org/10.1016/j.exer.2015.03.013
dc.identifier.citationExperimental Eye Research. London: Academic Press Ltd- Elsevier B.V., v. 134, p. 24-32, 2015.
dc.identifier.doi10.1016/j.exer.2015.03.013
dc.identifier.issn0014-4835
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/39044
dc.identifier.wosWOS:000354142000003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Eye Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectOcular allergyen
dc.subjectLipocortin 1en
dc.subjectTh1/Th2 cytokinesen
dc.subjectEosinophilsen
dc.subjectMast cellsen
dc.subjectERKen
dc.titleBeneficial effect of annexin A1 in a model of experimental allergic conjunctivitisen
dc.typeinfo:eu-repo/semantics/article
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