Sperm retrieval techniques in rats with suppressed spermatogenesis by experimental cryptorchidism

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dc.contributor.authorRossi, L. M.
dc.contributor.authorPereira, LAV
dc.contributor.authorSantis, L. de
dc.contributor.authorPasqualotto, F. F.
dc.contributor.authorIaconelli Júnior, Assumpto [UNIFESP]
dc.contributor.authorOrtiz, V
dc.contributor.authorBorges Júnior, Edson [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Caxias Sul
dc.date.accessioned2016-01-24T12:37:37Z
dc.date.available2016-01-24T12:37:37Z
dc.date.issued2005-02-01
dc.description.abstractObjective: Our aim was to assess the suppression of spermatogenesis and sperm retrieval rate after testicular sperm extraction (TESE) or testicular sperm aspiration (TESA) in adult rats with surgically induced cryptorchidism. Methods: Adult rats were submitted to TESE and TESA procedures after 15 days of induced cryptorchidism. After spermatozoa retrieval, the testicles were extracted, weighed and a morphological analysis by conventional light microscopy was done. the numbers of spermatozoa retrieved in both TESA and TESE were rated and compared. Results: Histological analysis of the testicles revealed Sertoli cell-only syndrome in 60% of the testicles, and maturation arrest in the remaining cryptorchid testicles. Significant differences were seen in the number of spermatozoa retrieved (P < 0.05) between cryptorchidic and control rats. When sperm retrieval techniques were compared, no differences were detected in the number of spermatozoa obtained (P > 0.05). Conclusions: It seems that a 15 day period of cryptorchidism is enough to induce spermatogenesis disorders. No differences were detected in the number of spermatozoa retrieved in the right or left testicles, irrespective of the testicular pole. Furthermore, and even more importantly, no differences in the retrieval rate were seen between the two techniques.en
dc.description.affiliationUniversidade Federal de São Paulo, Med Sch Jundiai, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Fertil Ctr, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Univ Estadual Campinas, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Urol, São Paulo, SP, Brazil
dc.description.affiliationUniv Caxias Sul, Dept Embriol, Caxias do Sul, RS, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Med Sch Jundiai, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fertil Ctr, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Univ Estadual Campinas, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Urol, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent443-447
dc.identifierhttp://dx.doi.org/10.1093/humrep/deh549
dc.identifier.citationHuman Reproduction. Oxford: Oxford Univ Press, v. 20, n. 2, p. 443-447, 2005.
dc.identifier.doi10.1093/humrep/deh549
dc.identifier.issn0268-1161
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28122
dc.identifier.wosWOS:000226611300019
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofHuman Reproduction
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectaspirationen
dc.subjectcryptorchidismen
dc.subjectextractionen
dc.subjectspermen
dc.subjecttesticleen
dc.titleSperm retrieval techniques in rats with suppressed spermatogenesis by experimental cryptorchidismen
dc.typeinfo:eu-repo/semantics/article
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