AC-1001 H3 CDR peptide induces apoptosis and signs of autophagy in vitro and exhibits antimetastatic activity in a syngeneic melanoma model

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dc.contributor.authorRabaca, Aline N. [UNIFESP]
dc.contributor.authorArruda, Denise C. [UNIFESP]
dc.contributor.authorFigueiredo, Carlos R. [UNIFESP]
dc.contributor.authorMassaoka, Mariana H. [UNIFESP]
dc.contributor.authorFarias, Camyla F. [UNIFESP]
dc.contributor.authorTada, Dayane B. [UNIFESP]
dc.contributor.authorMaia, Vera C.
dc.contributor.authorSilva Junior, Pedro I.
dc.contributor.authorGirola, Natalia [UNIFESP]
dc.contributor.authorReal, Fernando [UNIFESP]
dc.contributor.authorMortara, Renato A. [UNIFESP]
dc.contributor.authorPolonelli, Luciano
dc.contributor.authorTravassos, Luiz R. [UNIFESP]
dc.date.accessioned2019-07-22T15:46:46Z
dc.date.available2019-07-22T15:46:46Z
dc.date.issued2016
dc.description.abstractAntibody-derived peptides modulate functions of the immune system and are a source of anti-infective and antitumor substances. Recent studies have shown that they comprise amino acid sequences of immunoglobulin complementarity-determining regions, but also fragments of constant regions. V-H CDR3 of murine mAb AC-1001 displays antimetastatic activities using B16F10-Nex2 murine melanoma cells in a syngeneic model. The peptide was cytotoxic in vitro in murine and human melanoma cells inducing reactive oxygen species (ROS) and apoptosis by the intrinsic pathway. Signs of autophagy were also suggested by the increased expression of LC3/LC3II and Beclin 1 and by ultrastructural evidence. AC-1001 H3 bound to both G- and F-actin and inhibited tumor cell migration. These results are important evidence of the antitumor activity of Ig CDR-derived peptides.en
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Unidade Oncol Expt UNONEX, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Mogi das Cruzes, Nucleo Integrado Biotecnol, Mogi Das Cruzes, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Dept Ciencia & Tecnol, Sao Jose Dos Campos, Brazil
dc.description.affiliationRecepta Biopharma, Sao Paulo, Brazil
dc.description.affiliationInst Butantan, Lab Especial Toxinol Aplicada, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo UNIFESP, Dept Parasitol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Parma, Dept Biomed Biotechnol & Translat Sci, Microbiol & Virol Unit, I-43100 Parma, Italy
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Unidade Oncol Expt UNONEX, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Dept Ciencia & Tecnol, Sao Jose Dos Campos, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo UNIFESP, Dept Parasitol, Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010/16447-6, 51423-0]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipIDFAPESP:2010/16447-6
dc.description.sponsorshipID51423-0
dc.format.extent885-901
dc.identifierhttp://dx.doi.org/10.1002/2211-5463.12080
dc.identifier.citationFebs Open Bio. Hoboken, v. 6, n. 9, p. 885-901, 2016.
dc.identifier.doi10.1002/2211-5463.12080
dc.identifier.fileWOS000383618300002.pdf
dc.identifier.issn2211-5463
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51077
dc.identifier.wosWOS:000383618300002
dc.language.isoeng
dc.publisherWiley
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectapoptosisen
dc.subjectautophagyen
dc.subjectimmunoglobulin CDRen
dc.subjectmelanomaen
dc.subjectpeptidesen
dc.titleAC-1001 H3 CDR peptide induces apoptosis and signs of autophagy in vitro and exhibits antimetastatic activity in a syngeneic melanoma modelen
dc.typeinfo:eu-repo/semantics/article
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