Effects of simvastatin on cytokines secretion from mononuclear cells from critically ill patients with acute kidney injury

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dc.contributor.authorFerrari, Gabriela Laender [UNIFESP]
dc.contributor.authorQuinto, Beata Marie [UNIFESP]
dc.contributor.authorQueiroz, Kelly Cristina Batista de Souto [UNIFESP]
dc.contributor.authorIizuka, Ilson Jorge
dc.contributor.authorMonte, Julio Cesar Martins [UNIFESP]
dc.contributor.authorDalboni, Maria Aparecida [UNIFESP]
dc.contributor.authorDurão, Marcelino de Souza [UNIFESP]
dc.contributor.authorCendoroglo Neto, Miguel [UNIFESP]
dc.contributor.authorSantos, Oscar Fernando Pavão dos [UNIFESP]
dc.contributor.authorBatista, Marcelo Costa [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionIAEH IEP Hosp Israelita Albert Einstein Inst Ensi
dc.date.accessioned2016-01-24T14:06:26Z
dc.date.available2016-01-24T14:06:26Z
dc.date.issued2011-05-01
dc.description.abstractPurpose: To assess the in vitro effects of simvastatin on IL-10 and TNF-alpha secretion from peripheral blood mononuclear cells (PBMC) of critically ill patients with and without acute kidney injury (AKI).Methods: PBMC were collected from 63 patients admitted to the intensive care unit (ICU) and from 20 healthy controls. Patients were divided in 3 subgroups: with AKI, with sepsis and without AKI and with AKI and sepsis. After isolation by ficoll-gradient centrifugation cells were incubated in vitro with LPS 1 ng/mL, simvastatin (10(-8)M) and with LPS plus simvastatin for 24 h. TNF-alpha and IL-10 concentrations on cells surnatant were determined by ELISA.Results: Cells isolated from critically ill patients showed a decreased spontaneous production of TNF-alpha and IL-10 compared to healthy controls (6.7(0.2-12) vs 103(64-257) pg/mL and (20 (13-58) vs 315(105-510) pg/mL, respectively, p < 0.05). Under LPS-stimulus, IL-10 production remains lower in patients compared to healthy control (451 (176-850) vs 1150(874-1521) pg/mL,p < 0.05) but TNF-alpha production was higher (641 (609-841) vs 406 (201-841) pg/mL, p < 0.05). the simultaneous incubation with LPS and simvastatin caused decreased IL-10 production in cells from patients compared to control (337 (135-626) vs 540 (345-871) pg/mL, p < 0.05) and increased TNF-alpha release (711 (619-832) vs 324 (155-355) pg/mL, p < 0.05). Comparison between subgroups showed that the results observed in TNF-alpha and IL-10 production by PBMC from critically ill patients was independent of AKI occurrence.Conclusions: the PBMC treatment with simvastatin resulted in attenuation on pro-inflammatory cytokine spontaneous production that was no longer observed when these cells were submitted to a second inflammatory stimulus. Our study shows an imbalance between pro and anti-inflammatory cytokine production in PBMC from critically ill patients regardless the presence of AKI. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Nephrol, Dept Med, São Paulo, Brazil
dc.description.affiliationIAEH IEP Hosp Israelita Albert Einstein Inst Ensi, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nephrol, Dept Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipInstituto de Ensino e Pesquisa do Hospital Israelita Albert Einstein
dc.format.extent144-148
dc.identifierhttp://dx.doi.org/10.1016/j.cyto.2011.02.005
dc.identifier.citationCytokine. London: Academic Press Ltd- Elsevier B.V., v. 54, n. 2, p. 144-148, 2011.
dc.identifier.doi10.1016/j.cyto.2011.02.005
dc.identifier.fileWOS000290063200007.pdf
dc.identifier.issn1043-4666
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33646
dc.identifier.wosWOS:000290063200007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofCytokine
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectSimvastatinen
dc.subjectInflammationen
dc.subjectCytokinesen
dc.subjectSepsisen
dc.subjectAKIen
dc.titleEffects of simvastatin on cytokines secretion from mononuclear cells from critically ill patients with acute kidney injuryen
dc.typeinfo:eu-repo/semantics/article
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