Cyclooxygenase-2 expression in skeletal muscle of knockout mice suffering Duchenne muscular dystrophy

Página do item simplificado
dc.contributor.authorFlavia, De Oliveira [UNIFESP]
dc.contributor.authorQuintana, Hananiah Tardivo [UNIFESP]
dc.contributor.authorBortolin, Jeferson Andre [UNIFESP]
dc.contributor.authorGomes, Odair Alfredo
dc.contributor.authorLiberti, Edson Aparecido
dc.contributor.authorRibeiro, Daniel Araki [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Ribeirao Preto
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:31:40Z
dc.date.available2016-01-24T14:31:40Z
dc.date.issued2013-05-01
dc.description.abstractThe purpose of the present study was to investigate the role of cyclooxygenase-2 (COX-2) expression in fibrotic lesion in mdx mice. A total of six male C57BL/10 mice and six C57BL/10-DMD/mdx were distributed into two groups: control and animals with Duchenne muscular dystrophy (DMD). the medial part of gastrocnemius muscle was evaluated being the specimens stained with hematoxylin and eosin (H&E) and Sirius Red under normal and polarized light to differentiate type I (red and yellow) and III (green) collagen. COX-2 expression was assessed by immunohistochemistry. the results revealed histopathological changes in C57BL/10-DMD/mdx as depicted by regenerating fibers. Sirius Red stain showed a substantial increase in the amount of type I collagen of mdx mice. DMD induced a strong COX-2 immunoexpression in intercellular space. Taken together, our results are consistent with the notion that necrotic and fibrotic lesions are able to increase COX-2 expression in DMD.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biosci, UNIFESP, BR-11060001 Santos, SP, Brazil
dc.description.affiliationUniv Ribeirao Preto, UNAERP, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Anat, Inst Biomed Sci, ICB 3, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biosci, UNIFESP, BR-11060001 Santos, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent685-689
dc.identifierhttp://dx.doi.org/10.1007/s00418-012-1056-7
dc.identifier.citationHistochemistry and Cell Biology. New York: Springer, v. 139, n. 5, p. 685-689, 2013.
dc.identifier.doi10.1007/s00418-012-1056-7
dc.identifier.issn0948-6143
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36274
dc.identifier.wosWOS:000317688700005
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofHistochemistry and Cell Biology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectSkeletal muscleen
dc.subjectDuchenne muscular dystrophyen
dc.subjectConnective Tissueen
dc.subjectCyclooxygenase-2en
dc.subjectCollagenen
dc.titleCyclooxygenase-2 expression in skeletal muscle of knockout mice suffering Duchenne muscular dystrophyen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral