Interaction of medullary P2 and glutamate receptors mediates the vasodilation in the hindlimb of rat

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dc.contributor.authorKorim, Willian Seiji [UNIFESP]
dc.contributor.authorFerreira-Neto, Marcos L.
dc.contributor.authorPedrino, Gustavo R.
dc.contributor.authorPilowsky, Paul M.
dc.contributor.authorCravo, Sergio L. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionMacquarie Univ
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.date.accessioned2016-01-24T14:28:04Z
dc.date.available2016-01-24T14:28:04Z
dc.date.issued2012-12-01
dc.description.abstractIn the nucleus tractus solitarii (NTS) of rats, blockade of extracellular ATP breakdown to adenosine reduces arterial blood pressure (AP) increases that follow stimulation of the hypothalamic defense area (HDA). the effects of ATP on NTS P2 receptors, during stimulation of the HDA, are still unclear. the aim of this study was to determine whether activation of P2 receptors in the NTS mediates cardiovascular responses to HDA stimulation. Further investigation was taken to establish if changes in hindlimb vascular conductance (HVC) elicited by electrical stimulation of the HDA, or activation of P2 receptors in the NTS, are relayed in the rostral ventrolateral medulla (RVLM); and if those responses depend on glutamate release by ATP acting on presynaptic terminals. in anesthetized and paralyzed rats, electrical stimulation of the HDA increased AP and HVC. Blockade of P2 or glutamate receptors in the NTS, with bilateral microinjections of suramin (10 mM) or kynurenate (50 mM) reduced only the evoked increase in HVC by 75 % or more. Similar results were obtained with the blockade combining both antagonists. Blockade of P2 and glutamate receptors in the RVLM also reduced the increases in HVC to stimulation of the HDA by up to 75 %. Bilateral microinjections of kynurenate in the RVLM abolished changes in AP and HVC to injections of the P2 receptor agonist alpha,beta-methylene ATP (20 mM) into the NTS. the findings suggest that HDA-NTS-RVLM pathways in control of HVC are mediated by activation of P2 and glutamate receptors in the brainstem in alerting-defense reactions.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, Brazil
dc.description.affiliationMacquarie Univ, Australian Sch Adv Med, Sydney, NSW 2109, Australia
dc.description.affiliationUniv Fed Uberlandia, Fac Educ Fis, BR-38400 Uberlandia, MG, Brazil
dc.description.affiliationUniv Fed Goias, Dept Ciencias Fisiol, Goiania, Go, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipMacquarie Research Excellence Scholarship
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipNational Health and Medical Research Council of Australia
dc.description.sponsorshipAustralian Research Council
dc.description.sponsorshipGarnett Passe and Rodney Williams Memorial Foundation
dc.description.sponsorshipMacquarie University
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDNational Health and Medical Research Council of Australia: 457069
dc.description.sponsorshipIDNational Health and Medical Research Council of Australia: 457080
dc.description.sponsorshipIDNational Health and Medical Research Council of Australia: 604002
dc.description.sponsorshipIDAustralian Research Council: DP110102110
dc.description.sponsorshipIDCNPq: 477832/2010-5
dc.format.extent715-728
dc.identifierhttp://dx.doi.org/10.1007/s11302-012-9318-3
dc.identifier.citationPurinergic Signalling. Dordrecht: Springer, v. 8, n. 4, p. 715-728, 2012.
dc.identifier.doi10.1007/s11302-012-9318-3
dc.identifier.issn1573-9538
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35544
dc.identifier.wosWOS:000310221600007
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofPurinergic Signalling
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectHypothalamic defense areaen
dc.subjectRVLMen
dc.subjectNTSen
dc.subjectBlood flowen
dc.subjectATPen
dc.titleInteraction of medullary P2 and glutamate receptors mediates the vasodilation in the hindlimb of raten
dc.typeinfo:eu-repo/semantics/article
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