The anti-inflammatory action of Bothrops jararaca snake antithrombin on acute inflammation induced by carrageenan in mice

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dc.contributor.authorMorais-Zani, Karen de
dc.contributor.authorBarbosa Nunes, Fernanda Peixoto
dc.contributor.authorSilva, Jacilene Barbosa da
dc.contributor.authorFerreira, Marcio Jose
dc.contributor.authorGrego, Kathleen Fernandes
dc.contributor.authorLopes-Ferreira, Monica
dc.contributor.authorTanaka, Aparecida Sadae [UNIFESP]
dc.contributor.authorTanaka-Azevedo, Anita Mitico
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:32:03Z
dc.date.available2016-01-24T14:32:03Z
dc.date.issued2013-08-01
dc.description.abstractAntithrombin is known as the most important natural coagulation inhibitor and has been shown to have anti-inflammatory properties. the present study aimed to investigate the effects of Bothrops jararaca antithrombin on acute inflammation induced by carrageenan in mice.We evaluated the anti-inflammatory activity of antithrombin on models of paw edema formation, cell migration and leukocyte-endothelium interaction in mice (Swiss; n = 5). Acute inflammation was induced by the administration of carrageenan (15 mg kg(-1)).Treatment with B. jararaca antithrombin (1 mg kg(-1)) 1 h before or after carrageenan administration significantly inhibited paw edema formation, reduced cell influx to the peritoneal cavity due to reduction in the migration of polymorphonuclear cells, and attenuated leukocyte rolling in the microcirculation of the cremaster muscle.The effects of antithrombin on vascular and cellular events of inflammation were completely abolished by treatment with the cyclo-oxygenase inhibitor indomethacin (4 mg kg(-1)), suggesting the involvement of prostacyclin in the mechanism of inflammation inhibition by B. jararaca antithrombin.This work showed for the first time the anti-inflammatory properties of B. jararaca antithrombin on vascular and cellular events of inflammation. These findings suggest that antithrombin is effective in preventing paw edema formation, cell migration and leukocyte rolling induced by carrageenan in mice.en
dc.description.affiliationInst Butantan, Lab Herpetol, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Programa Posgrad Interunidades Biotecnol, Inst Pesquisas Tecnol, Inst Butantan, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Fisiol, Inst Biociencias, BR-05508900 São Paulo, Brazil
dc.description.affiliationInst Butantan, CEPID FAPESP, Lab Especial Toxinolgia Aplicada, BR-05503900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 09/50199-2
dc.description.sponsorshipIDFAPESP: 09/03484-3
dc.description.sponsorshipIDFAPESP: 11/21558-4
dc.format.extent733-742
dc.identifierhttp://dx.doi.org/10.1007/s00011-013-0628-x
dc.identifier.citationInflammation Research. Basel: Springer Basel Ag, v. 62, n. 8, p. 733-742, 2013.
dc.identifier.doi10.1007/s00011-013-0628-x
dc.identifier.issn1023-3830
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36580
dc.identifier.wosWOS:000321867800001
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofInflammation Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectAntithrombinen
dc.subjectBothrops jararacaen
dc.subjectAcute inflammationen
dc.subjectAnti-inflammatory activityen
dc.titleThe anti-inflammatory action of Bothrops jararaca snake antithrombin on acute inflammation induced by carrageenan in miceen
dc.typeinfo:eu-repo/semantics/article
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