Physicochemical and chromatographic evaluation of benzhydrylamine-resin as an anion exchanger solid support

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dc.contributor.authorCarvalho, Regina Siqueira Haddad [UNIFESP]
dc.contributor.authorNakaie, Clovis Ryuichi [UNIFESP]
dc.contributor.authorSilva, Elias Horacio da [UNIFESP]
dc.contributor.authorSantos, Isabel Anunciacao Neves dos [UNIFESP]
dc.contributor.authorDietrich, Carl Peter von [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorTersariol, Ivarne Luis dos Santos [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:33:57Z
dc.date.available2016-01-24T12:33:57Z
dc.date.issued2003-07-31
dc.description.abstractBenzhydrylamine-resin (BHAR), a copolymer of styrene-divinylbenzene containing phenylmethylamine groups, used as solid support for peptide synthesis, was examined regarding physicochemical and anion exchanger chromatographic properties. the greater the ionic strength of the medium the poorer the solvation of beads. This effect is less pronounced the higher the amino group content of BHAR. the BHAR chromatographic behavior was compared with commercial cationic resins in columns of constant cation binding capacity. Three negatively charged heparan sulfate disaccharides were successfully purified in a 2.4 mmol/g BHAR that showed as good or better anion exchange performance than classical tertiary or quaternary amino group-containing resins. the BHAR chromatographic resin exclusion limit was estimated to be 30 kDa based on purification experiments of heparins of different molecular weight. (C) 2003 Elsevier Science B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent75-83
dc.identifierhttps://dx.doi.org/10.1016/S0165-022X(03)00104-0
dc.identifier.citationJournal of Biochemical and Biophysical Methods. Amsterdam: Elsevier B.V., v. 57, n. 1, p. 75-83, 2003.
dc.identifier.doi10.1016/S0165-022X(03)00104-0
dc.identifier.issn0165-022X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/27326
dc.identifier.wosWOS:000184300900007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Biochemical and Biophysical Methods
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectIon exchanger chromatographyen
dc.subjectPolymeren
dc.subjectDisaccharideen
dc.subjectBenzhydrylamine-resinen
dc.subjectBenzydrylamineen
dc.subjectPeptideen
dc.titlePhysicochemical and chromatographic evaluation of benzhydrylamine-resin as an anion exchanger solid supporten
dc.typeinfo:eu-repo/semantics/article
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