Novel model for calcium paradox in sympathetic transmission of smooth muscles: Role of cyclic AMP pathway

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dc.contributor.authorBergantin, Leandro Bueno [UNIFESP]
dc.contributor.authorSouza, Claudio Fontes [UNIFESP]
dc.contributor.authorFerreira, Regiane Miranda [UNIFESP]
dc.contributor.authorSmaili, Soraya Soubhi [UNIFESP]
dc.contributor.authorJurkiewicz, Neide Hyppolito [UNIFESP]
dc.contributor.authorCaricati-Neto, Afonso [UNIFESP]
dc.contributor.authorJurkiewicz, Aron [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:34:21Z
dc.date.available2016-01-24T14:34:21Z
dc.date.issued2013-09-01
dc.description.abstractIt is well established that reduction of Ca2+ influx through L-type voltage-dependent Ca2+ channel (L-type VDCC), or increase of cytosolic cAMP concentration ([cAMP]c), inhibit contractile activity of smooth muscles in response to transmitters released from sympathetic nerves. Surprisingly, in this work we observed that simultaneous administration of L-type VDCC blocker (verapamil) and [cAMP]c enhancers (rolipram, IBMX and forskolin) potentiated purinergic contractions evoked by electrical field stimulation of rat vas deferens, instead of inhibiting them. These results, including its role in sympathetic transmission, can be considered as a calcium paradox. On the other hand, this potentiation was prevented by reduction of [cAMP]c by inhibition of adenylyl cyclase (SQ 22536) or depletion of Ca2+ storage of sarco-endoplasmic reticulum by blockade of Ca2+ reuptake (thapsigargin). in addition, cytosolic concentration ([Ca2+](c)) evaluated by fluorescence microscopy in rat adrenal medullary slices was significantly reduced by verapamil or rolipram. in contrast, simultaneous incubation of adrenal slices with these compounds significantly increased [Ca2+](c). This effect was prevented by thapsigargin. Thus, a reduction of [Ca2+](c) due to blockade of Ca2+ influx through L-type VDCC could stimulate adenylyl cyclase activity increasing [cAMP]c thereby stimulating Ca2+ release from endoplasmic reticulum, resulting in augmented transmitter release in sympathetic nerves and contraction. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, INFAR, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Func Lab, Res Ctr 2, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, INFAR, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Func Lab, Res Ctr 2, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent202-212
dc.identifierhttp://dx.doi.org/10.1016/j.ceca.2013.06.004
dc.identifier.citationCell Calcium. Edinburgh: Churchill Livingstone, v. 54, n. 3, p. 202-212, 2013.
dc.identifier.doi10.1016/j.ceca.2013.06.004
dc.identifier.issn0143-4160
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36708
dc.identifier.wosWOS:000324721300007
dc.language.isoeng
dc.publisherChurchill Livingstone
dc.relation.ispartofCell Calcium
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCalciumen
dc.subjectcAMPen
dc.subjectSympathetic transmissionen
dc.subjectSmooth muscleen
dc.titleNovel model for calcium paradox in sympathetic transmission of smooth muscles: Role of cyclic AMP pathwayen
dc.typeinfo:eu-repo/semantics/article
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