Mapping and validation of xanthomonas citri subsp citri genes regulated by putative plant-inducible promoter box (pip-box)

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dc.contributor.authorCarvalho, F. M. S.
dc.contributor.authorOliveira, J. C. F. [UNIFESP]
dc.contributor.authorLaia, M. L.
dc.contributor.authorJacob, T. R.
dc.contributor.authorFerreira, R. M.
dc.contributor.authorFerro, M. I. T.
dc.contributor.authorTezza, R. I. D.
dc.contributor.authorZingaretti, S. M.
dc.contributor.authorSilva, C. F.
dc.contributor.authorFerro, J. A.
dc.date.accessioned2019-01-21T10:29:47Z
dc.date.available2019-01-21T10:29:47Z
dc.date.issued2016
dc.description.abstractCitrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp citri (Xac), is a major disease affecting citriculture worldwide, because of the susceptibility of the host and the lack of efficient control methods. Previous studies have reported that some genes of phytopathogenic bacteria possess a consensus nucleotide sequence (TTCGC...N15...TTCGC) designated the "plant-inducible-promoter box" (PIP box) located in the promoter region, which is responsible for activating the expression of pathogenicity and virulence factors when the pathogen is in contact with the host plant. In this study, we mapped and investigated the expression of 104 Xac genes associated with the PIP box sequences using a macroarray analysis. Xac gene expression was observed during in vitro (Xac grown for 12 or 20 h in XAM1 induction medium) or in vivo (bacteria grown in orange leaves for 3 to 5 days) infection conditions. Xac grown in non-induction NB liquid medium was used as the control. cDNA was isolated from bacteria grown under the different conditions and hybridized to the macroarray, and 32 genes differentially expressed during the infection period (in vitro or in vivo induction) were identified. The macroarray results were validated for some of the genes through semi-quantitative RT-PCR, and the functionality of the PIP box-containing promoter was demonstrated by activating beta-glucuronidase reporter gene activity by the PIP box-containing promoter region during Xac-citrus host interaction.en
dc.description.affiliationLaboratório de Bioquímica e Biologia Molecular, Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista,Jaboticabal, SP, Brasil
dc.description.affiliationDepartamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil
dc.description.affiliationDepartamento de Ciências Biológicas, Universidade Federal de São Paulo, Diadema, SP, Brasil
dc.description.affiliationDepartamento de Engenharia Florestal, Faculdade de Ciências Agrárias, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, MG, Brasil
dc.description.affiliationUnidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, Brasil
dc.description.affiliationCentro de Recursos Biológicos e Biologia Genômica, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brasil
dc.description.affiliationUnifespDepartamento de Ciências Biológicas, Universidade Federal de São Paulo, Diadema, SP, Brasil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipCoodernacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.format.extentUNSP 15028428
dc.identifierhttp://dx.doi.org/10.4238/gmr.15028428
dc.identifier.citationGenetics And Molecular Research. Ribeirao preto, v. 15, n. 2, p. UNSP 15028428, 2016.
dc.identifier.doi10.4238/gmr.15028428
dc.identifier.issn1676-5680
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49388
dc.identifier.wosWOS:000384881000120
dc.language.isoeng
dc.publisherDove Medical Press Ltd
dc.relation.ispartofGenetics And Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPip-Box Promoteren
dc.subjectGene Reporter Beta-Glucuronidaseen
dc.subjectCitrus Cankeren
dc.subjectXanthomonas Citrien
dc.subjectXami Induction MediumCampestris Pv Vesicatoriaen
dc.subjectIii Secretion Systemen
dc.subjectRalstonia-Solanacearumen
dc.subjectMycobacterium-Tuberculosisen
dc.subjectExpressionen
dc.subjectSequenceen
dc.subjectIdentificationen
dc.subjectVirulenceen
dc.subjectOryzaeen
dc.subjectActivationen
dc.titleMapping and validation of xanthomonas citri subsp citri genes regulated by putative plant-inducible promoter box (pip-box)en
dc.typeinfo:eu-repo/semantics/article
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